CLIP-170 Antibody (F-3): sc-28325

CLIP-170 Antibody (F-3) is a mouse monoclonal IgG2b kappa light chain antibody that detects CLIP-170 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). CLIP-170 (F-3) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The cytoplasmic linker protein (CLIP-170) plays a crucial role in cellular dynamics as a member of the plus-end-binding proteins (+TIPs), which are essential for microtubule stabilization and organization. By binding to the growing plus ends of microtubules, CLIP-170 acts as a linker between these dynamic structures and organelle membranes, thereby promoting microtubule rescue and preventing catastrophe, particularly near the cell periphery. This function is vital for maintaining cellular integrity and facilitating intracellular transport, as CLIP-170 ensures that microtubules remain stable and functional during cellular processes such as mitosis and organelle movement. Additionally, fluorescently labeled CLIP-170 can be visualized as comet-like streaks at the growing ends of microtubules, providing insights into dynamic behavior in live-cell imaging studies. CLIP-170 also co-localizes with Dynactin and Dynein at microtubule ends and at the kinetochore, highlighting CLIP-170′s importance in the orchestration of cellular transport and division. Furthermore, CLIP-170 is highly expressed in Hodgkin and Reed-Sternberg (HRS) cells, monocyte-derived dendritic cells, interleukin-4 plus CD40 ligand activated B cells, and Ki-1 lymphoma, making CLIP-170 a significant marker in various pathological conditions.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CLIP-170 Antibody (F-3) References:

1. CLIP-170 highlights growing microtubule ends in vivo.  |  Perez, F., et al. 1999. Cell. 96: 517-27. PMID: 10052454
2. The cytoplasmic linker protein CLIP-170 is a human autoantigen.  |  Griffith, KJ., et al. 2002. Clin Exp Immunol. 127: 533-8. PMID: 11966772
3. Hodgkin and Reed-Sternberg cell-associated autoantigen CLIP-170/restin is a marker for dendritic cells and is involved in the trafficking of macropinosomes to the cytoskeleton, supporting a function-based concept of Hodgkin and Reed-Sternberg cells.  |  Sahin, U., et al. 2002. Blood. 100: 4139-45. PMID: 12433698
4. Cytoplasmic linker proteins promote microtubule rescue in vivo.  |  Komarova, YA., et al. 2002. J Cell Biol. 159: 589-99. PMID: 12446741
5. CLIP-170 interacts with dynactin complex and the APC-binding protein EB1 by different mechanisms.  |  Goodson, HV., et al. 2003. Cell Motil Cytoskeleton. 55: 156-73. PMID: 12789661
6. CLIP-170 links endocytic vesicles to microtubules.  |  Pierre, P., et al. 1992. Cell. 70: 887-900. PMID: 1356075
7. Minimal plus-end tracking unit of the cytoplasmic linker protein CLIP-170.  |  Gupta, KK., et al. 2009. J Biol Chem. 284: 6735-42. PMID: 19074770
8. Cytoplasmic Linker Protein CLIP170 Negatively Regulates TLR4 Signaling by Targeting the TLR Adaptor Protein TIRAP.  |  Jakka, P., et al. 2018. J Immunol. 200: 704-714. PMID: 29222167
9. Dynactin 1 negatively regulates HIV-1 infection by sequestering the host cofactor CLIP170.  |  Shanmugapriya, S., et al. 2021. Proc Natl Acad Sci U S A. 118: PMID: 34686593
10. Pregnenolone-methyl-ether enhances CLIP170 and microtubule functions improving spine maturation and hippocampal deficits related to CDKL5 deficiency.  |  Barbiero, I., et al. 2022. Hum Mol Genet. 31: 2738-2750. PMID: 35348691
11. CLIP170 inhibits the metastasis and EMT of papillary thyroid cancer through the TGF-β pathway.  |  Ma, B., et al. 2024. Med Oncol. 41: 137. PMID: 38705933
12. Restin in Hodgkin's disease and anaplastic large cell lymphoma.  |  Delabie, J., et al. 1993. Leuk Lymphoma. 12: 21-6. PMID: 8161933

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CLIP-170 Antibody (F-3) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-CLIP-170 Antibody (F-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CLIP-170 Antibody (F-3) Specifications

Alternate Product Name: CLIP1

Product Clone Name: F-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 1128-1427

Epitope Target: CLIP-170

Epitope Species: human

Product Reactivity/Detection: CLIP-170

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for CLIP-170 Antibody (F-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin