CLIP-170 Antibody (E-8): sc-166801

CLIP-170 Antibody (E-8) is a mouse monoclonal IgG3 antibody that detects CLIP-170 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CLIP-170, a crucial member of the plus-end-binding proteins (+TIPs), plays a significant role in microtubule dynamics by binding to the growing plus ends of microtubules and linking them to organelle membranes. This interaction is vital for maintaining cellular structure and function, as CLIP-170 acts as an anticatastrophic factor, promoting microtubule rescue, particularly near the cell periphery, which is essential for processes such as cell division and intracellular transport. CLIP-170′s unique ability to form comet-like structures at microtubule ends allows for real-time visualization of microtubule dynamics, providing insights into cellular behavior. Additionally, CLIP-170 co-localizes with key proteins such as Dynactin and Dynein at microtubule ends and the kinetochore, highlighting CLIP-170′s importance in the orchestration of cellular transport and mitotic processes. Notably, the splice variant Restin, which includes a 35 amino acid stretch absent in CLIP-170, is highly expressed in Hodgkin and Reed-Sternberg cells, monocyte-derived dendritic cells, IL-4 + CD40L activated B cells, and Ki-1 lymphoma, further underscoring CLIP-170′s relevance in various biological contexts.

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CLIP-170 Antibody (E-8) References:

1. Colocalization of cytoplasmic dynein with dynactin and CLIP-170 at microtubule distal ends.  |  Vaughan, KT., et al. 1999. J Cell Sci. 112 (Pt 10): 1437-47. PMID: 10212138
2. Purification and analysis of authentic CLIP-170 and recombinant fragments.  |  Scheel, J., et al. 1999. J Biol Chem. 274: 25883-91. PMID: 10464331
3. Conformational changes in CLIP-170 regulate its binding to microtubules and dynactin localization.  |  Lansbergen, G., et al. 2004. J Cell Biol. 166: 1003-14. PMID: 15381688
4. Microtubule-binding protein CLIP-170 is a mediator of paclitaxel sensitivity.  |  Sun, X., et al. 2012. J Pathol. 226: 666-73. PMID: 21989536
5. Microtubule plus end-associated CLIP-170 initiates HSV-1 retrograde transport in primary human cells.  |  Jovasevic, V., et al. 2015. J Cell Biol. 211: 323-37. PMID: 26504169
6. Phosphorylation of EB1 regulates the recruitment of CLIP-170 and p150glued to the plus ends of astral microtubules.  |  Ran, J., et al. 2017. Oncotarget. 8: 9858-9867. PMID: 28039481
7. Stress-induced phosphorylation of CLIP-170 by JNK promotes microtubule rescue.  |  Henrie, H., et al. 2020. J Cell Biol. 219: PMID: 32491151
8. SOCS3-microtubule interaction via CLIP-170 and CLASP2 is critical for modulation of endothelial inflammation and lung injury.  |  Karki, P., et al. 2021. J Biol Chem. 296: 100239. PMID: 33372035
9. LIS1 interacts with CLIP170 to promote tumor growth and metastasis via the Cdc42 signaling pathway in salivary gland adenoid cystic carcinoma.  |  Li, L., et al. 2022. Int J Oncol. 61: PMID: 36102310
10. Alf1p, a CLIP-170 domain-containing protein, is functionally and physically associated with alpha-tubulin.  |  Feierbach, B., et al. 1999. J Cell Biol. 144: 113-24. PMID: 9885248

The preferred CLIP-170 antibody is CLIP-170 Antibody (F-3): sc-28325.