CLIM-2 Double Nickase Plasmid (m): sc-421402-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CLIM-2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CLIM-2 Double Nickase Plasmid (m) and CLIM-2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Ldb1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CLIM-2 Antibody (C-9): sc-365074
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CLIM-2 Double Nickase Plasmid (m) | sc-421402-NIC | 20 µg | $410.00 | |||
CLIM-2 Double Nickase Plasmid (m2) | sc-421402-NIC-2 | 20 µg | $410.00 |
LIM domain-binding protein 1 (Ldb1), also known as CLIM-2, is a nuclear adaptor that bridges LIM-homeodomain and LIM-only transcription factors to assemble higher-order regulatory complexes. In mouse cells, Ldb1 helps coordinate lineage-specific transcriptional programs that shape embryonic patterning, hematopoietic differentiation, and neuronal development by stabilizing enhancer–promoter communication. Through interactions with factors such as LHX proteins and LMO cofactors, it modulates chromatin-associated control of gene expression and cell fate decisions. Dysregulation of Ldb1-centered transcriptional networks is relevant to mechanisms of aberrant differentiation and oncogenic transcriptional circuitry, making it a useful target for dissecting developmental and disease-associated regulatory pathways.
CLIM-2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Ldb1 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Ldb1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Ldb1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Ldb1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.