CLIM-2 Antibody (F-11): sc-365281

CLIM-2 Antibody (F-11) is a mouse monoclonal IgM antibody that detects CLIM-2 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CLIM-2, also known as nuclear LIM interactor, plays a crucial role in forming transcriptional regulatory complexes by interacting with LIM-only proteins like LMO2 and LMO4, which are essential for gene expression regulation in various cellular contexts. CLIM-2 is primarily located in the nucleus, where CLIM-2 facilitates the assembly of heteromeric LIM complexes, enhancing nuclear retention of LIM proteins and promoting transcriptional activity. This localization ensures LIM proteins can effectively regulate gene expression in response to cellular signals, influencing processes such as cell differentiation and development. CLIM-2′s interaction with other proteins in the LIM family underscores CLIM-2′s importance in maintaining the delicate balance of gene regulation necessary for proper cellular function and development. anti-CLIM-2 antibody (F-11) is an invaluable tool for researchers studying the intricate networks of transcriptional regulation and the role of LIM proteins in various biological processes.

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CLIM-2 Antibody (F-11) References:

1. Genomic structure, alternative transcripts and chromosome location of the human LIM domain binding protein 1 gene LDB1.  |  Drechsler, M., et al. 1999. Cytogenet Cell Genet. 87: 119-24. PMID: 10640831
2. The podocyte-specific inactivation of Lmx1b, Ldb1 and E2a yields new insight into a transcriptional network in podocytes.  |  Suleiman, H., et al. 2007. Dev Biol. 304: 701-12. PMID: 17316599
3. LDB1 overexpression is a negative prognostic factor in colorectal cancer.  |  García, SA., et al. 2016. Oncotarget. 7: 84258-84270. PMID: 27713177
4. Crystal structure of human LDB1 in complex with SSBP2.  |  Wang, H., et al. 2020. Proc Natl Acad Sci U S A. 117: 1042-1048. PMID: 31892537
5. Polymer Thin Film Promotes Tumor Spheroid Formation via JAK2-STAT3 Signaling Primed by Fibronectin-Integrin α5 and Sustained by LMO2-LDB1 Complex.  |  Seo, S., et al. 2022. Biomedicines. 10: PMID: 36359204
6. An intricate regulatory circuit between FLI1 and GATA1/GATA2/LDB1/ERG dictates erythroid vs. megakaryocytic differentiation.  |  Wang, C., et al. 2024. Mol Med Rep. 29: PMID: 38695236
7. Enhancer looping protein LDB1 modulates MYB expression in T-ALL cell lines in vitro by cooperating with master transcription factors.  |  Li, Y., et al. 2024. J Exp Clin Cancer Res. 43: 283. PMID: 39385230
8. The LIM-domain-only protein LMO2 and its binding partner LDB1 are differentially required for class switch recombination.  |  Yang, B., et al. 2025. Proc Natl Acad Sci U S A. 122: e2412376122. PMID: 39847321
9. The LIM-domain binding protein Ldb1 and its partner LMO2 act as negative regulators of erythroid differentiation.  |  Visvader, JE., et al. 1997. Proc Natl Acad Sci U S A. 94: 13707-12. PMID: 9391090
10. Interactions between LIM domains and the LIM domain-binding protein Ldb1.  |  Breen, JJ., et al. 1998. J Biol Chem. 273: 4712-7. PMID: 9468533

The preferred CLIM-2 antibody is CLIM-2 Antibody (C-9): sc-365074.