CLIC5B Antibody (D-10): sc-514066
- CLIC5B Antibody (D-10) is a mouse monoclonal IgG1 κ CLIC5B antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 30-54 near the N-terminus of CLIC5B of human origin
- CLIC5B Antibody (D-10) is recommended for detection of CLIC5B of human origin by WB, IP, IF and ELISA
- Anti-CLIC5B Antibody (D-10) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP and m-IgG1 BP-HRP are the preferred secondary detection reagents for CLIC5B Antibody (D-10) for WB applications. These reagents are now offered in bundles with CLIC5B Antibody (D-10) (see ordering information below).
CLIC5B Antibody (D-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects CLIC5B protein of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CLIC5B (D-10) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Chloride intracellular channel 5 (CLIC5) is a vital member of a highly conserved family of chloride ion channels that exist in both soluble and integral membrane forms, playing a crucial role in regulating cellular traffic of chloride ions, which are essential for maintaining cellular homeostasis. The proper functioning of chloride channels is critical for stabilizing membrane potential, facilitating signal transduction, regulating cell volume, and transporting organic solutes. CLIC5 is particularly important in the assembly and maintenance of actin-based cytoskeletal structures, and CLIC5 has been implicated in inner ear function, where CLIC5 localizes to stereocilia and may interact with Radixin. Notably, CLIC5 exists in two isoforms, CLIC5A and CLIC5B, which share an identical C-terminus but have distinct N-terminal sequences. CLIC5B, the human ortholog of bovine p64 and avian p62, binds to AKAP350 and is localized to the Golgi apparatus, highlighting CLIC5B′s significance in cellular organization and signaling pathways.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CLIC5B Antibody (D-10) References:
- Identification of a novel member of the chloride intracellular channel gene family (CLIC5) that associates with the actin cytoskeleton of placental microvilli. | Berryman, M. and Bretscher, A. 2000. Mol Biol Cell. 11: 1509-21. PMID: 10793131
- Differential expression of a chloride intracellular channel gene, CLIC4, in transforming growth factor-beta1-mediated conversion of fibroblasts to myofibroblasts. | Rønnov-Jessen, L., et al. 2002. Am J Pathol. 161: 471-80. PMID: 12163372
- AKAP350 at the Golgi apparatus. II. Association of AKAP350 with a novel chloride intracellular channel (CLIC) family member. | Shanks, RA., et al. 2002. J Biol Chem. 277: 40973-80. PMID: 12163479
- Identification of a novel member of the CLIC family, CLIC6, mapping to 21q22.12. | Friedli, M., et al. 2003. Gene. 320: 31-40. PMID: 14597386
- CLIC-5A functions as a chloride channel in vitro and associates with the cortical actin cytoskeleton in vitro and in vivo. | Berryman, M., et al. 2004. J Biol Chem. 279: 34794-801. PMID: 15184393
- Silencing of Peroxiredoxin 2 and aberrant methylation of 33 CpG islands in putative promoter regions in human malignant melanomas. | Furuta, J., et al. 2006. Cancer Res. 66: 6080-6. PMID: 16778180
- c-Src control of chloride channel support for osteoclast HCl transport and bone resorption. | Edwards, JC., et al. 2006. J Biol Chem. 281: 28011-22. PMID: 16831863
- The chloride intracellular channel protein CLIC5 is expressed at high levels in hair cell stereocilia and is essential for normal inner ear function. | Gagnon, LH., et al. 2006. J Neurosci. 26: 10188-98. PMID: 17021174
- Expression and cellular localisation of chloride intracellular channel 3 in human placenta and fetal membranes. | Money, TT., et al. 2007. Placenta. 28: 429-36. PMID: 17027078
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CLIC5B Antibody (D-10) | sc-514066 | 200 µg/ml | $322.00 | |||
CLIC5B Antibody (D-10): m-IgG Fc BP-HRP Bundle | sc-527847 | 200 µg Ab; 10 µg BP | $361.00 | |||
CLIC5B Antibody (D-10): m-IgG1 BP-HRP Bundle | sc-533220 | 200 µg Ab; 20 µg BP | $361.00 | |||
CLIC5B Antibody (D-10) AC | sc-514066 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CLIC5B Antibody (D-10) HRP | sc-514066 HRP | 200 µg/ml | $322.00 | |||
CLIC5B Antibody (D-10) FITC | sc-514066 FITC | 200 µg/ml | $336.00 | |||
CLIC5B Antibody (D-10) PE | sc-514066 PE | 200 µg/ml | $349.00 | |||
CLIC5B Antibody (D-10) Alexa Fluor® 488 | sc-514066 AF488 | 200 µg/ml | $364.00 | |||
CLIC5B Antibody (D-10) Alexa Fluor® 546 | sc-514066 AF546 | 200 µg/ml | $364.00 | |||
CLIC5B Antibody (D-10) Alexa Fluor® 594 | sc-514066 AF594 | 200 µg/ml | $364.00 | |||
CLIC5B Antibody (D-10) Alexa Fluor® 647 | sc-514066 AF647 | 200 µg/ml | $364.00 | |||
CLIC5B Antibody (D-10) Alexa Fluor® 680 | sc-514066 AF680 | 200 µg/ml | $364.00 | |||
CLIC5B Antibody (D-10) Alexa Fluor® 790 | sc-514066 AF790 | 200 µg/ml | $364.00 | |||
CLIC5B (D-10) Neutralizing Peptide | sc-514066 P | 100 µg/0.5 ml | $69.00 |