CLIC4 Antibody (45.42): sc-135739

CLIC4 Antibody (45.42) is a mouse monoclonal IgG1 kappa light chain antibody that detects CLIC4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Anti-CLIC4 antibody (45.42) is available in both non-conjugated and various conjugated forms, including agarose and horseradish peroxidase (HRP). CLIC4, also known as chloride intracellular channel 4, H1, huH1, p64H1, CLIC4L, or MTCLIC, is a 253 amino acid single-pass membrane protein that plays a crucial role in cellular ion transport. CLIC4 is primarily localized in the nucleus and cytoplasm and features a GST C-terminal domain, which is significant for function. CLIC4 is expressed in various tissues, including the placenta, heart, skeletal muscle, and epithelial cells from the kidney, colon, and esophagus, where CLIC4 facilitates the selective transport of chloride and other ions across membranes. This ion transport is vital for maintaining cellular homeostasis and regulating physiological processes such as cell volume, pH balance, and electrical excitability. The gene encoding CLIC4 is located on human chromosome 1, a region that spans 260 million base pairs and contains over 3,000 genes, many of which are associated with various diseases, including familial adenomatous polyposis, Stickler syndrome, and several cancers. Understanding the function and localization of CLIC4 is essential for elucidating its role in health and disease, making CLIC4 (45.42) antibody a valuable tool for researchers studying ion channel dynamics and related pathologies.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CLIC4 Antibody (45.42) References:

1. A novel p64-related Cl- channel: subcellular distribution and nephron segment-specific expression.  |  Edwards, JC. 1999. Am J Physiol. 276: F398-408. PMID: 10070163
2. A 29 kDa intracellular chloride channel p64H1 is associated with large dense-core vesicles in rat hippocampal neurons.  |  Chuang, JZ., et al. 1999. J Neurosci. 19: 2919-28. PMID: 10191309
3. p53 and tumor necrosis factor alpha regulate the expression of a mitochondrial chloride channel protein.  |  Fernández-Salas, E., et al. 1999. J Biol Chem. 274: 36488-97. PMID: 10593946
4. mtCLIC/CLIC4, an organellular chloride channel protein, is increased by DNA damage and participates in the apoptotic response to p53.  |  Fernández-Salas, E., et al. 2002. Mol Cell Biol. 22: 3610-20. PMID: 11997498
5. Proteomic analysis of vascular endothelial growth factor-induced endothelial cell differentiation reveals a role for chloride intracellular channel 4 (CLIC4) in tubular morphogenesis.  |  Bohman, S., et al. 2005. J Biol Chem. 280: 42397-404. PMID: 16239224
6. Reciprocal modifications of CLIC4 in tumor epithelium and stroma mark malignant progression of multiple human cancers.  |  Suh, KS., et al. 2007. Clin Cancer Res. 13: 121-31. PMID: 17200346
7. CLIC4, skin homeostasis and cutaneous cancer: surprising connections.  |  Suh, KS., et al. 2007. Mol Carcinog. 46: 599-604. PMID: 17443730
8. CLIC4 mediates and is required for Ca2+-induced keratinocyte differentiation.  |  Suh, KS., et al. 2007. J Cell Sci. 120: 2631-40. PMID: 17636002
9. Functional reconstitution of mammalian 'chloride intracellular channels' CLIC1, CLIC4 and CLIC5 reveals differential regulation by cytoskeletal actin.  |  Singh, H., et al. 2007. FEBS J. 274: 6306-16. PMID: 18028448
10. CLIC4 abrogation promotes epithelial-mesenchymal transition in gastric cancer.  |  Wang, B., et al. 2020. Carcinogenesis. 41: 841-849. PMID: 31560739
11. CLIC4 localizes to mitochondrial-associated membranes and mediates cardioprotection.  |  Ponnalagu, D., et al. 2022. Sci Adv. 8: eabo1244. PMID: 36269835
12. CLIC4 Regulates Endothelial Barrier Control by Mediating PAR1 Signaling via RhoA.  |  Kleinjan, ML., et al. 2023. Arterioscler Thromb Vasc Biol. 43: 1441-1454. PMID: 37317855

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CLIC4 Antibody (45.42) has 13 citations in a variety of scientific publications.

Primary antibodies like Anti-CLIC4 Antibody (45.42) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CLIC4 Antibody (45.42) Specifications

Alternate Product Name: CLIC4

Product Clone Name: 45.42

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 238-250

Epitope Target: CLIC4

Epitope Species: human

Product Reactivity/Detection: CLIC4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 13 citations in various scientific publications

Available Conjugates/Forms for CLIC4 Antibody (45.42): AC: Agarose, HRP: Horseradish Peroxidase

The preferred CLIC4/5/6 antibody is CLIC4/5/6 Antibody (A-11): sc-271863.