PARP1 Antibody (194C1439): sc-56196

PARP1 Antibody (194C1439) is an IgG2b mouse monoclonal PARP1 antibody (also designated PARP-1 antibody or poly(ADP-ribose) polymerase 1 antibody) that detects the cleaved PARP-1 protein of mouse, rat and human origin by WB and IP. Poly(ADP-ribose) polymerase 1 (PARP1) is a nuclear enzyme that plays a key role in DNA repair, chromatin remodeling, and cell survival. Upon detecting DNA strand breaks, PARP1 rapidly binds to damaged sites and catalyzes the transfer of ADP-ribose units from NAD⁺ to target proteins, a process known as PARylation, which facilitates the recruitment of DNA repair factors. During apoptosis, PARP1 is specifically cleaved by caspase-3 and caspase-7 at a conserved site, producing an 89 kDa C-terminal fragment containing the catalytic domain and a 24 kDa N-terminal fragment containing the DNA-binding domain. This cleavage inactivates PARP1, preventing it from consuming cellular NAD⁺ and ATP during irreversible cell damage. Studying cleaved PARP1 is important because its presence serves as a hallmark of apoptosis, making it a widely used biomarker in cancer research, drug screening, and studies of neurodegeneration and immune responses. Detection of cleaved PARP1 can provide a deeper understanding into the efficacy of pro-apoptotic therapies and help distinguish apoptotic from necrotic or viable cells.

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Contains Mercury: All HRP conjugates (including bundle forms) contain thiomersal, a mercury compound. Do not dispose in trash. Dispose as hazardous chemical waste according to local, state and federal regulations.

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Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PARP1 Antibody (194C1439) has 131 citations in a variety of scientific publications.

Primary antibodies like Anti-PARP1 Antibody (194C1439) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PARP1 Antibody (194C1439) Specifications

Alternate Product Name: PARP-1

Product Clone Name: 194C1439

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b

Amino Acids:

Epitope Target: PARP1

Epitope Species: human

Product Reactivity/Detection: cleavad product of PARP-1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 50 µg IgG_2b in 0.5 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 131 citations in various scientific publications

Available Conjugates/Forms for PARP1 Antibody (194C1439): N/A

The preferred PARP1 antibody is PARP1 Antibody (F-2): sc-8007.