claudin-4 Antibody (A-12): sc-376643

Claudin-4 Antibody (A-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects claudin-4 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-claudin-4 antibody (A-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Claudin-4 plays a crucial role in maintaining the integrity of tight junctions, which are essential for regulating paracellular transport and maintaining the barrier function of epithelial and endothelial tissues. These tight junctions prevent solute leakage and maintain distinct environments of different tissue compartments. Claudin-4 is specifically localized to tight junctions in various tissues, including the pancreas, where claudin-4 is found at the junctions of ductal and acinar cells, and in the colon, where claudin-4 is expressed in surface epithelial cells. The human claudin-4 gene is located on chromosome 7q11.23, and claudin-4 expression is tightly regulated, highlighting its importance in tissue-specific functions and the overall maintenance of cellular homeostasis.

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claudin-4 Antibody (A-12) References:

1. Transmembrane proteins in the tight junction barrier.  |  Fanning, AS., et al. 1999. J Am Soc Nephrol. 10: 1337-45. PMID: 10361874
2. Clostridium perfringens enterotoxin binds to the second extracellular loop of claudin-3, a tight junction integral membrane protein.  |  Fujita, K., et al. 2000. FEBS Lett. 476: 258-61. PMID: 10913624
3. Heterogeneity in expression and subcellular localization of claudins 2, 3, 4, and 5 in the rat liver, pancreas, and gut.  |  Rahner, C., et al. 2001. Gastroenterology. 120: 411-22. PMID: 11159882
4. The roles of claudin superfamily proteins in paracellular transport.  |  Heiskala, M., et al. 2001. Traffic. 2: 93-8. PMID: 11247307
5. Interleukin-2 receptor beta subunit-dependent and -independent regulation of intestinal epithelial tight junctions.  |  Nishiyama, R., et al. 2001. J Biol Chem. 276: 35571-80. PMID: 11466322
6. Regulation of claudin-4 via p63 in human epithelial cells.  |  Kojima, T., et al. 2017. Ann N Y Acad Sci. 1405: 25-31. PMID: 28856683
7. The role of claudin-4 in the development of gastric cancer.  |  Liu, W. and Li, M. 2020. Scand J Gastroenterol. 55: 1072-1078. PMID: 32715822
8. Comparison of claudin-3 and claudin-4 expression in bilateral and unilateral breast cancer.  |  Szade, J., et al. 2021. Neoplasma. 68: 283-289. PMID: 33147053
9. Claudin-4 Immunoexpression in Urothelial Carcinomas.  |  SĂndulescu, AŞ., et al. 2020. Curr Health Sci J. 46: 379-382. PMID: 33717512
10. Potential roles of claudin-3 and claudin-4 in ovarian cancer management.  |  Uthayanan, L. and El-Bahrawy, M. 2022. J Egypt Natl Canc Inst. 34: 24. PMID: 35665865
11. Claudin-4 localization in epithelial ovarian cancer.  |  Neville, MC., et al. 2022. Heliyon. 8: e10862. PMID: 36237976
12. Expression of Claudin-4 and D2-40 and their significance in prostatic adenocarcinoma.  |  Kabra, H., et al. 2023. J Cancer Res Ther. 19: S800-S806. PMID: 38384059

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-claudin-4 Antibody (A-12) has 30 citations in a variety of scientific publications.

Primary antibodies like Anti-claudin-4 Antibody (A-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

claudin-4 Antibody (A-12) Specifications

Alternate Product Name: claudin-4

Product Clone Name: A-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 183-209

Epitope Target: claudin-4

Epitope Species: human

Product Reactivity/Detection: claudin-4

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 30 citations in various scientific publications

Available Conjugates/Forms for claudin-4 Antibody (A-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin