CLASP2 Double Nickase Plasmid (h): sc-403609-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CLASP2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CLASP2 Double Nickase Plasmid (h) and CLASP2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CLASP2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CLASP2 Antibody (F-3): sc-376496
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CLASP2 Double Nickase Plasmid (h) | sc-403609-NIC | 20 µg | $410.00 | |||
CLASP2 Double Nickase Plasmid (h2) | sc-403609-NIC-2 | 20 µg | $410.00 |
CLASP2 (cytoplasmic linker–associated protein 2) is a microtubule plus-end tracking protein that stabilizes and organizes microtubules at the cell cortex and kinetochores, supporting spindle assembly, chromosome segregation, and directional cell migration. By coupling microtubules to cortical and adhesion-associated complexes, CLASP2 contributes to cytoskeletal crosstalk that influences cell polarity and mitotic fidelity. Its regulation intersects with signaling networks that control microtubule dynamics and attachment geometry, including pathways governing spindle positioning and centrosome function. Dysregulated CLASP2 activity or expression has been linked to aberrant mitosis and altered migratory behavior, making it relevant for studies of genomic instability and proliferative phenotypes in disease-relevant cellular models.
CLASP2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CLASP2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CLASP2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CLASP2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CLASP2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.