CIRP Double Nickase Plasmid (h): sc-405185-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CIRP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CIRP Double Nickase Plasmid (h) and CIRP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CIRBP. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CIRP Antibody (1C9): sc-293325
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CIRP Double Nickase Plasmid (h) | sc-405185-NIC | 20 µg | $410.00 | |||
CIRP Double Nickase Plasmid (h2) | sc-405185-NIC-2 | 20 µg | $410.00 |
Cold-inducible RNA-binding protein (CIRP), encoded by CIRBP, is a stress-responsive RNA chaperone that modulates mRNA stability, translation, and subcellular localization in response to hypothermia, UV irradiation, and oxidative stress. CIRP participates in post-transcriptional regulatory networks that influence cell-cycle control, apoptosis, and inflammatory signaling, including stress granule dynamics and broader RNA metabolism pathways. Altered CIRBP expression or localization has been associated with dysregulated stress adaptation and immune responses, with relevance to cancer biology, neuroinflammation, and ischemia-related cellular injury. As a multifunctional RNA-binding protein, CIRP provides a tractable node for dissecting how environmental stress reshapes gene expression programs at the RNA level.
CIRP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CIRBP locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CIRBP. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CIRBP function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CIRBP-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.