CHT1 Antibody (62-2ES): sc-33713
- CHT1 Antibody (62-2ES) is a mouse monoclonal IgG1 κ CHT1 antibody, cited in 5 publications, provided at 200 µg/ml
- raised against recombinant protein from the C-terminus of human CHT
- CHT1 Antibody (62-2ES) is recommended for detection of CHT1 of mouse, rat and human origin by WB, IP and IF
- Anti-CHT1 Antibody (62-2ES) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for CHT1 Antibody (62-2ES) for WB applications. This reagent is now offered in a bundle with CHT1 Antibody (62-2ES) (see ordering information below).
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SEE ALSO...
CHT1 Antibody (62-2ES) is a mouse monoclonal IgG1 kappa light chain antibody that detects CHT1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). CHT1 (62-2ES) antibody is available in non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. The choline transporter 1 (CHT1) plays a crucial role in cholinergic neurotransmission by mediating high-affinity choline uptake, essential for acetylcholine synthesis. This process is particularly important in cholinergic neurons, where choline availability directly influences acetylcholine production and release. CHT1 is predominantly expressed in regions of the nervous system rich in cholinergic cell bodies, such as the spinal cord, brainstem, mid-brain, and striatum, while remaining absent in tissues like the cerebellum and kidney that lack cholinergic cells. CHT1 localizes to specific small vesicles within cholinergic presynaptic terminals, which also contain vesicular acetylcholine transporter and acetylcholine, highlighting functional significance. These vesicles move to the plasma membrane in response to neuronal activity, facilitating choline re-uptake and potentially coupling this process with acetylcholine release, thereby influencing synaptic transmission and plasticity.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CHT1 Antibody (62-2ES) References:
- Molecular cloning of a human, hemicholinium-3-sensitive choline transporter. | Apparsundaram, S., et al. 2000. Biochem Biophys Res Commun. 276: 862-7. PMID: 11027560
- Functional characterization of the human high-affinity choline transporter. | Okuda, T. and Haga, T. 2000. FEBS Lett. 484: 92-7. PMID: 11068039
- Molecular cloning and characterization of a murine hemicholinium-3-sensitive choline transporter. | Apparsundaram, S., et al. 2001. Biochem Soc Trans. 29: 711-6. PMID: 11709061
- Expression of the high-affinity choline transporter, CHT1, in the neuronal and non-neuronal cholinergic system of human and rat skin. | Haberberger, RV., et al. 2002. J Invest Dermatol. 119: 943-8. PMID: 12406342
- Expression of the high-affinity choline transporter CHT1 in epithelia. | Pfeil, U., et al. 2003. Life Sci. 72: 2087-90. PMID: 12628461
- The hemicholinium-3 sensitive high affinity choline transporter is internalized by clathrin-mediated endocytosis and is present in endosomes and synaptic vesicles. | Ribeiro, FM., et al. 2003. J Neurochem. 87: 136-46. PMID: 12969261
- Vesicular localization and activity-dependent trafficking of presynaptic choline transporters. | Ferguson, SM., et al. 2003. J Neurosci. 23: 9697-709. PMID: 14585997
- Reduction in CHT1-mediated choline uptake in primary neurons from presenilin-1 M146V mutant knock-in mice. | Payette, DJ., et al. 2007. Brain Res. 1135: 12-21. PMID: 17196556
- The high-affinity choline transporter CHT1 is regulated by the ubiquitin ligase Nedd4-2. | Yamada, H., et al. 2012. Biomed Res. 33: 1-8. PMID: 22361880
- Molecular properties of the high-affinity choline transporter CHT1. | Haga, T. 2014. J Biochem. 156: 181-94. PMID: 25073461
- Activation of the High-Affinity Choline Transporter 1 in the Spinal Cord Relieves Stress-Induced Hyperalgesia. | Lin, M., et al. 2023. Dig Dis Sci. 68: 2414-2426. PMID: 36808325
- Transport mechanism of presynaptic high-affinity choline uptake by CHT1. | Qiu, Y., et al. 2024. Nat Struct Mol Biol. 31: 701-709. PMID: 38589607
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CHT1 Antibody (62-2ES) | sc-33713 | 200 µg/ml | $316.00 | |||
CHT1 Antibody (62-2ES): m-IgG Fc BP-HRP Bundle | sc-525547 | 200 µg Ab; 10 µg BP | $354.00 | |||
CHT1 Antibody (62-2ES) AC | sc-33713 AC | 500 µg/ml, 25% agarose | $416.00 | |||
CHT1 Antibody (62-2ES) HRP | sc-33713 HRP | 200 µg/ml | $316.00 | |||
CHT1 Antibody (62-2ES) FITC | sc-33713 FITC | 200 µg/ml | $330.00 | |||
CHT1 Antibody (62-2ES) PE | sc-33713 PE | 200 µg/ml | $343.00 | |||
CHT1 Antibody (62-2ES) Alexa Fluor® 488 | sc-33713 AF488 | 200 µg/ml | $357.00 | |||
CHT1 Antibody (62-2ES) Alexa Fluor® 546 | sc-33713 AF546 | 200 µg/ml | $357.00 | |||
CHT1 Antibody (62-2ES) Alexa Fluor® 594 | sc-33713 AF594 | 200 µg/ml | $357.00 | |||
CHT1 Antibody (62-2ES) Alexa Fluor® 647 | sc-33713 AF647 | 200 µg/ml | $357.00 | |||
CHT1 Antibody (62-2ES) Alexa Fluor® 680 | sc-33713 AF680 | 200 µg/ml | $357.00 | |||
CHT1 Antibody (62-2ES) Alexa Fluor® 790 | sc-33713 AF790 | 200 µg/ml | $357.00 |