Chr-A Antibody (C-12): sc-393941

Chr-A Antibody (C-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects Chromogranin A (Chr-A) in mouse, rat, and human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). Chr-A (C-12) antibody is available in both non-conjugated and multiple conjugated forms, such as agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, providing versatility for different experimental needs. Chr-A is an important acidic glycoprotein primarily located within secretory granules of neuroendocrine and endocrine cells, playing a crucial role in hormone and neuropeptide storage and release. Chr-A presence is particularly significant in neuroendocrine tumors, where elevated levels can serve as a biomarker for diagnosis and monitoring. Chr-A is also the precursor for several biologically active peptides, including pancreastatin, which regulates insulin and gastric acid secretion, highlighting Chr-A importance in metabolic processes. Chr-A interaction with other proteins, such as carboxypeptidase E, further underscores Chr-A role in neuropeptide processing and secretion, making Chr-A a vital component in the neuroendocrine system.

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Chr-A Antibody (C-12) References:

1. Influence of spinal cord transection on the presence and axonal transport of CGRP-, chromogranin A-, VIP-, synapsin I-, and synaptophysin-like immunoreactivities in rat motor nerve.  |  Li, JY., et al. 1992. J Neurobiol. 23: 1094-110. PMID: 1281222
2. Chromogranin A immunoreactivity compared with argyrophilia, calcitonin immunoreactivity, and amyloid as tumour markers in the histopathological diagnosis of medullary (C-cell) thyroid carcinoma.  |  Harach, HR., et al. 1992. Pathol Res Pract. 188: 123-30. PMID: 1594480
3. Chromogranin A as a marker of neuroendocrine histogenesis of tumours: an immunoelectron microscopic study with considerations about the influence of fixation and embedding media on immunolabelling.  |  Leone, BE., et al. 1990. Basic Appl Histochem. 34: 143-53. PMID: 2222401
4. Rapid axonal transport as a chromatographic process: the use of immunocytochemistry of ligated nerves to investigate the biochemistry of anterogradely versus retrogradely transported organelles.  |  Dahlström, AB. and Bööj, S. 1988. Cell Motil Cytoskeleton. 10: 309-20. PMID: 2460258
5. Plasma chromogranin-A in primary hyperparathyroidism.  |  Nanes, MS., et al. 1989. J Clin Endocrinol Metab. 69: 950-5. PMID: 2571619
6. [Chromogranin A derived peptide CGA47-66 inhibits hyper-permeability of blood brain barrier in mice with sepsis].  |  Zeng, Y., et al. 2016. Zhonghua Wei Zhong Bing Ji Jiu Yi Xue. 28: 122-6. PMID: 26911943
7. Chrysomycin A Attenuates Neuroinflammation by Down-Regulating NLRP3/Cleaved Caspase-1 Signaling Pathway in LPS-Stimulated Mice and BV2 Cells.  |  Liu, M., et al. 2021. Int J Mol Sci. 22: PMID: 34202695
8. Chrysomycin A Inhibits the Proliferation, Migration and Invasion of U251 and U87-MG Glioblastoma Cells to Exert Its Anti-Cancer Effects.  |  Liu, DN., et al. 2022. Molecules. 27: PMID: 36234681
9. Chrysomycin A Regulates Proliferation and Apoptosis of Neuroglioma Cells via the Akt/GSK-3β Signaling Pathway In Vivo and In Vitro.  |  Liu, DN., et al. 2023. Mar Drugs. 21: PMID: 37367654
10. Chrysomycin A Reshapes Metabolism and Increases Oxidative Stress to Hinder Glioblastoma Progression.  |  Liu, DN., et al. 2024. Mar Drugs. 22: PMID: 39330272

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Chr-A Antibody (C-12) has 48 citations in a variety of scientific publications.

Primary antibodies like Anti-Chr-A Antibody (C-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Chr-A Antibody (C-12) Specifications

Alternate Product Name: Chromogranin A

Product Clone Name: C-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 442-457

Epitope Target: Chr-A

Epitope Species: human

Product Reactivity/Detection: Chr-A and Chr-A derived peptides ER-37 and GR-44

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: equine

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 48 citations in various scientific publications

Available Conjugates/Forms for Chr-A Antibody (C-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, P: Blocking Peptide, PE: Phycoerythrin