CHD5 Antibody (D-10): sc-271248

CHD5 Antibody (D-10) is a mouse monoclonal IgG1 kappa light chain antibody that detects CHD5 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CHD5 Antibody (D-10) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Chromodomain helicase DNA binding protein 5 (CHD5) is a crucial member of the SNF2/RAD54 helicase family, which is integral to chromatin remodeling and DNA binding processes. CHD5 is predominantly expressed in the fetal and adult brain, where CHD5 plays a vital role in nervous system development and functions as a tumor suppressor through the Arf/p53 pathway. CHD5 is involved in the formation of remodeling complexes, such as the NuRD complex, which is essential for normal neuroblast maturation and helps prevent neuronal cell overexpression. The structural integrity of CHD5 is critical, as errors in chromatin remodeling can lead to uncontrolled cell growth, inhibiting differentiation and potentially resulting in tumor formation. Deletions in the gene encoding CHD5 are frequently observed in neuroblastomas, indicating that CHD5 deficiency may contribute to malignant cell transformation and metastasis, underscoring CHD5′s significance in maintaining proper brain development and function.

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CHD5 Antibody (D-10) References:

1. CHD5 defines a new subfamily of chromodomain-SWI2/SNF2-like helicases.  |  Schuster, EF. and Stöger, R. 2002. Mamm Genome. 13: 117-9. PMID: 11889561
2. CHD5, a new member of the chromodomain gene family, is preferentially expressed in the nervous system.  |  Thompson, PM., et al. 2003. Oncogene. 22: 1002-11. PMID: 12592387
3. Cooperative function of the CHD5-like protein Mdm39p with a P-type ATPase Spf1p in the maintenance of ER homeostasis in Saccharomyces cerevisiae.  |  Ando, A. and Suzuki, C. 2005. Mol Genet Genomics. 273: 497-506. PMID: 15909163
4. The consensus coding sequences of human breast and colorectal cancers.  |  Sjöblom, T., et al. 2006. Science. 314: 268-74. PMID: 16959974
5. CHD5 is a tumor suppressor at human 1p36.  |  Bagchi, A., et al. 2007. Cell. 128: 459-75. PMID: 17289567
6. The Chd family of chromatin remodelers.  |  Marfella, CG. and Imbalzano, AN. 2007. Mutat Res. 618: 30-40. PMID: 17350655
7. Mutual regulation between CHD5 and EZH2 in hepatocellular carcinoma.  |  Xie, CR., et al. 2015. Oncotarget. 6: 40940-52. PMID: 26517514
8. Missense and truncating variants in CHD5 in a dominant neurodevelopmental disorder with intellectual disability, behavioral disturbances, and epilepsy.  |  Parenti, I., et al. 2021. Hum Genet. 140: 1109-1120. PMID: 33944996
9. CHD5 inhibits metastasis of neuroblastoma.  |  Laut, AK., et al. 2022. Oncogene. 41: 622-633. PMID: 34789839
10. Pan-Cancer Analysis Identifies CHD5 as a Potential Biomarker for Glioma.  |  Xu, L., et al. 2022. Int J Mol Sci. 23: PMID: 35955624
11. Chd5 Regulates the Transcription Factor Six3 to Promote Neuronal Differentiation.  |  Shrestha, P., et al. 2023. Stem Cells. 41: 242-251. PMID: 36636025
12. Expression and sequence analysis of candidates for the 1p36.31 tumor suppressor gene deleted in neuroblastomas.  |  Okawa, ER., et al. 2008. Oncogene. 27: 803-10. PMID: 17667943

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CHD5 Antibody (D-10) has 4 citations in a variety of scientific publications.

Primary antibodies like Anti-CHD5 Antibody (D-10) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CHD5 Antibody (D-10) Specifications

Alternate Product Name: CHD5

Product Clone Name: D-10

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1521-1705

Epitope Target: CHD5

Epitope Species: human

Product Reactivity/Detection: CHD5

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 4 citations in various scientific publications

Available Conjugates/Forms for CHD5 Antibody (D-10): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent