CHCHD3 CRISPR/Cas9 KO Plasmid (m): sc-425804
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CHCHD3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CHCHD3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CHCHD3 CRISPR/Cas9 KO Plasmid (m) | sc-425804 | 20 µg | $397.00 |
Overview
Chchd3 encodes CHCHD3 (also known as Mic19), a core component of the mitochondrial contact site and cristae organizing system (MICOS) that maintains inner mitochondrial membrane architecture and cristae junction integrity. By scaffolding protein assemblies at cristae junctions and coordinating with mitochondrial dynamics and protein import machineries, CHCHD3 supports oxidative phosphorylation efficiency, respiratory chain organization, and mitochondrial quality control. Disruption of MICOS components, including CHCHD3, is linked to altered bioenergetics, increased reactive oxygen species, and downstream impacts on apoptosis, mitophagy, and cellular stress signaling. These processes are frequently interrogated in models of neurodegeneration, cardiometabolic dysfunction, and cancer cell metabolic adaptation where mitochondrial ultrastructure and respiration are central phenotypes.
CHCHD3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Chchd3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Chchd3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Chchd3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CHCHD3 protein expression.
This CRISPR knockout system enables efficient generation of Chchd3-deficient cell models for investigation of CHCHD3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Chchd3 exon(s) critical for CHCHD3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Chchd3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CHCHD3 CRISPR/Cas9 KO Plasmid (m) and CHCHD3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Chchd3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CHCHD3 HDR Plasmid (m) and CHCHD3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Chchd3 homology arms to support homology-directed repair at defined Chchd3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.