CGNL1 Antibody (E-6): sc-377525

CGNL1 Antibody (E-6) is a mouse monoclonal IgG1 kappa light chain antibody that detects CGNL1 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-CGNL1 antibody (E-6) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CGNL1, also known as cingulin-like 1 or junction-associated coiled-coil protein, is a critical component of tight junctions, which are essential for maintaining the integrity of epithelial and endothelial barriers. CGNL1 consists of 1,302 amino acids and plays a vital role in anchoring the apical junctional complex to the actin-based cytoskeleton, thereby regulating the permeability of paracellular pathways and preventing the diffusion of solutes across cellular barriers. CGNL1 is expressed in various tissues, including smooth muscle, spleen, testis, and several regions of the adult brain, such as the amygdala and cerebellum. CGNL1 exists as two alternatively spliced isoforms and is encoded by a gene located on human chromosome 15q21.3. Mutations in the CGNL1 gene have been linked to aromatase excess syndrome, a condition characterized by elevated estrogen levels due to increased aromatase activity, highlighting CGNL1 ′s significance in hormonal regulation and cellular function.

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CGNL1 Antibody (E-6) References:

1. Cingulin interacts with F-actin in vitro.  |  D'Atri, F. and Citi, S. 2001. FEBS Lett. 507: 21-4. PMID: 11682052
2. Evidence for a functional interaction between cingulin and ZO-1 in cultured cells.  |  D'Atri, F., et al. 2002. J Biol Chem. 277: 27757-64. PMID: 12023291
3. Estrogen excess associated with novel gain-of-function mutations affecting the aromatase gene.  |  Shozu, M., et al. 2003. N Engl J Med. 348: 1855-65. PMID: 12736278
4. Establishment and characterization of cultured epithelial cells lacking expression of ZO-1.  |  Umeda, K., et al. 2004. J Biol Chem. 279: 44785-94. PMID: 15292177
5. JACOP, a novel plaque protein localizing at the apical junctional complex with sequence similarity to cingulin.  |  Ohnishi, H., et al. 2004. J Biol Chem. 279: 46014-22. PMID: 15292197
6. Disruption of the cingulin gene does not prevent tight junction formation but alters gene expression.  |  Guillemot, L., et al. 2004. J Cell Sci. 117: 5245-56. PMID: 15454572
7. Histone deacetylase inhibitors up-regulate the expression of tight junction proteins.  |  Bordin, M., et al. 2004. Mol Cancer Res. 2: 692-701. PMID: 15634758
8. Binding of GEF-H1 to the tight junction-associated adaptor cingulin results in inhibition of Rho signaling and G1/S phase transition.  |  Aijaz, S., et al. 2005. Dev Cell. 8: 777-86. PMID: 15866167
9. Regional rearrangements in chromosome 15q21 cause formation of cryptic promoters for the CYP19 (aromatase) gene.  |  Demura, M., et al. 2007. Hum Mol Genet. 16: 2529-41. PMID: 17584767

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CGNL1 Antibody (E-6) has 2 citations in a variety of scientific publications.

Primary antibodies like Anti-CGNL1 Antibody (E-6) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CGNL1 Antibody (E-6) Specifications

Alternate Product Name: CGNL1

Product Clone Name: E-6

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 770-957

Epitope Target: CGNL1

Epitope Species: human

Product Reactivity/Detection: CGNL1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 2 citations in various scientific publications

Available Conjugates/Forms for CGNL1 Antibody (E-6): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin