CES1 Double Nickase Plasmid (h): sc-405773-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CES1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CES1 Double Nickase Plasmid (h) and CES1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CES1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CES1 Antibody (A-11): sc-365249
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CES1 Double Nickase Plasmid (h) | sc-405773-NIC | 20 µg | $410.00 | |||
CES1 Double Nickase Plasmid (h2) | sc-405773-NIC-2 | 20 µg | $410.00 |
Carboxylesterase 1 (CES1) is a serine hydrolase highly expressed in human liver that catalyzes the hydrolysis of a broad range of endogenous lipids and xenobiotic esters, amides, and thioesters. By regulating the turnover of neutral lipids and esterified metabolites, CES1 contributes to lipid homeostasis and intersects with metabolic and detoxification processes that shape cellular redox balance and inflammatory signaling. Altered CES1 activity or expression has been associated with inter-individual variability in drug metabolism and with metabolic phenotypes linked to dyslipidemia and fatty liver-related pathology. As a result, CES1 is widely used as a molecular handle to study hepatic metabolism, chemical biotransformation, and mechanisms underlying variable compound response in human systems.
CES1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CES1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CES1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CES1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CES1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.