Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
ceruloplasmin CRISPR/Cas9 KO Plasmid (h): sc-401798
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- ceruloplasmin CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the ceruloplasmin genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: ceruloplasmin Antibody (H-3): sc-365205
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
ceruloplasmin CRISPR/Cas9 KO Plasmid (h) | sc-401798 | 20 µg | $397.00 |
Overview
CP encodes ceruloplasmin, a copper-binding multicopper oxidase that catalyzes Fe²⁺ to Fe³⁺ oxidation to support transferrin loading and systemic iron distribution. It is a key component of copper homeostasis and ferroxidase activity, linking hepatic secretion and extracellular redox control to iron trafficking and oxidative stress responses. Ceruloplasmin function intersects with pathways regulating iron export, reactive oxygen species balance, and inflammatory signaling in tissues with high metabolic demand. Dysregulated CP activity is associated with altered iron handling and oxidative damage phenotypes, making it a useful target for studying mechanisms relevant to neurodegeneration, liver physiology, and anemia-related cellular stress models.
ceruloplasmin CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CP gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CP together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CP open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish ceruloplasmin protein expression.
This CRISPR knockout system enables efficient generation of CP-deficient cell models for investigation of ceruloplasmin signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CP exon(s) critical for ceruloplasmin function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CP genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by ceruloplasmin CRISPR/Cas9 KO Plasmid (h) and ceruloplasmin CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CP locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by ceruloplasmin HDR Plasmid (h) and ceruloplasmin HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CP homology arms to support homology-directed repair at defined CP target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.