Cerebellin 1 CRISPR/Cas9 KO Plasmid (h): sc-407032
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cerebellin 1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Cerebellin 1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cerebellin 1 CRISPR/Cas9 KO Plasmid (h) | sc-407032 | 20 µg | $397.00 |
Overview
CBLN1 encodes cerebellin 1, a secreted glycoprotein enriched in the central nervous system that acts as a synaptic organizer supporting excitatory synapse formation and maintenance. Cerebellin 1 participates in trans-synaptic adhesion complexes that couple presynaptic and postsynaptic specializations, shaping synaptic connectivity and neurotransmission in cerebellar and related circuits. Through these interactions it influences neuronal development, synaptic plasticity, and circuit refinement processes central to motor coordination and learning. Altered CBLN1 expression or disrupted cerebellin-mediated synaptic organization has been associated with neurodevelopmental and neuropsychiatric phenotypes, making it relevant for mechanistic studies of synaptopathies.
Cerebellin 1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CBLN1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CBLN1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CBLN1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Cerebellin 1 protein expression.
This CRISPR knockout system enables efficient generation of CBLN1-deficient cell models for investigation of Cerebellin 1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CBLN1 exon(s) critical for Cerebellin 1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CBLN1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Cerebellin 1 CRISPR/Cas9 KO Plasmid (h) and Cerebellin 1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CBLN1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Cerebellin 1 HDR Plasmid (h) and Cerebellin 1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CBLN1 homology arms to support homology-directed repair at defined CBLN1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.