CEP70 CRISPR/Cas9 KO Plasmid (h): sc-406527
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CEP70 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CEP70 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CEP70 CRISPR/Cas9 KO Plasmid (h) | sc-406527 | 20 µg | $397.00 |
Overview
CEP70 (centrosomal protein 70) is a centrosome-associated factor implicated in centrosome maturation and microtubule organization during cell cycle progression. By supporting centrosomal architecture and spindle function, CEP70 contributes to efficient mitotic entry, chromosome segregation, and the maintenance of cellular polarity. Perturbation of centrosome and microtubule regulatory networks is linked to genomic instability and altered proliferative capacity, making CEP70 relevant to studies of mitotic fidelity and cytoskeletal control. Dysregulated centrosome function and spindle dynamics are frequently observed in cancer-related contexts, positioning CEP70 as a useful node for investigating mechanisms that couple centrosome homeostasis to cell proliferation.
CEP70 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CEP70 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CEP70 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CEP70 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CEP70 protein expression.
This CRISPR knockout system enables efficient generation of CEP70-deficient cell models for investigation of CEP70 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CEP70 exon(s) critical for CEP70 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CEP70 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CEP70 CRISPR/Cas9 KO Plasmid (h) and CEP70 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CEP70 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CEP70 HDR Plasmid (h) and CEP70 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CEP70 homology arms to support homology-directed repair at defined CEP70 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.