CENPJ Antibody (E-12): sc-166113

CENPJ Antibody (E-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects CENPJ of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). anti-CENPJ antibody (E-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CENPJ, also known as CPAP, is crucial for proper brain development as CENPJ plays a significant role in regulating brain size. CENPJ is characterized by five coiled-coil domains, with the most C-terminal domain featuring a leucine zipper motif, which is important for protein-protein interactions. Additionally, CENPJ contains several potential phosphorylation sites and a C-terminal domain with 21 nonamer G-box repeats, indicating CENPJ′s involvement in various signaling pathways. CENPJ gene expression in neuroepithelium during prenatal neurogenesis and CENPJ localization to spindle poles during mitosis suggest a central role in the centrosomal mechanisms that control neuron number in the developing mammalian brain. Mutations in CENPJ have been associated with autosomal recessive primary microcephaly, with a specific 4 base pair deletion identified as a cause of protein truncation, leading to this condition.

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CENPJ Antibody (E-12) References:

1. Protein 4.1 R-135 interacts with a novel centrosomal protein (CPAP) which is associated with the gamma-tubulin complex.  |  Hung, LY., et al. 2000. Mol Cell Biol. 20: 7813-25. PMID: 11003675
2. CPAP is a novel stat5-interacting cofactor that augments stat5-mediated transcriptional activity.  |  Peng, B., et al. 2002. Mol Endocrinol. 16: 2019-33. PMID: 12198240
3. LAG-3: a regulator of T-cell and DC responses and its use in therapeutic vaccination.  |  Triebel, F. 2003. Trends Immunol. 24: 619-22. PMID: 14644131
4. Identification of a novel microtubule-destabilizing motif in CPAP that binds to tubulin heterodimers and inhibits microtubule assembly.  |  Hung, LY., et al. 2004. Mol Biol Cell. 15: 2697-706. PMID: 15047868
5. A centrosomal mechanism involving CDK5RAP2 and CENPJ controls brain size.  |  Bond, J., et al. 2005. Nat Genet. 37: 353-5. PMID: 15793586
6. Depletion of CPAP by RNAi disrupts centrosome integrity and induces multipolar spindles.  |  Cho, JH., et al. 2006. Biochem Biophys Res Commun. 339: 742-7. PMID: 16316625
7. Molecular evolution of the brain size regulator genes CDK5RAP2 and CENPJ.  |  Evans, PD., et al. 2006. Gene. 375: 75-9. PMID: 16631324
8. What primary microcephaly can tell us about brain growth.  |  Cox, J., et al. 2006. Trends Mol Med. 12: 358-66. PMID: 16829198
9. A novel deletion mutation in CENPJ gene in a Pakistani family with autosomal recessive primary microcephaly.  |  Gul, A., et al. 2006. J Hum Genet. 51: 760-764. PMID: 16900296

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CENPJ Antibody (E-12) has 1 citations in a variety of scientific publications.

Primary antibodies like Anti-CENPJ Antibody (E-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CENPJ Antibody (E-12) Specifications

Alternate Product Name: CENPJ

Product Clone Name: E-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-300

Epitope Target: CENPJ

Epitope Species: human

Product Reactivity/Detection: CENPJ

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 1 citations in various scientific publications

Available Conjugates/Forms for CENPJ Antibody (E-12): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin