Anti-CENP-H Antibody (G-9) recommended for detection of CENP-H of human origin by WB, IP, IF, IHC(P) and ELISA

Anti-CENP-H Antibody (G-9): sc-365222

Anti-CENP-H Antibody (G-9) is rated 5.0 out of 5 by 2.
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Datasheets

    • Anti-CENP-H Antibody (G-9) is a mouse monoclonal IgG2a κ CENP-H antibody, cited in 1 publications, provided at 200 µg/ml
    • raised against amino acids 1-247 representing full length CENP-H of human origin
    • CENP-H Antibody (G-9) is recommended for detection of CENP-H of human origin by WB, IP, IF, IHC(P) and ELISA
    • Anti-CENP-H Antibody (G-9) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
    • also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
    • Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of CENP-H (G-9): sc-365222.
    • m-IgG Fc BP-HRP is the preferred secondary detection reagent for CENP-H Antibody (G-9) for WB and IHC(P) applications. This reagent is now offered in a bundle with CENP-H Antibody (G-9) (see ordering information below).
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Can CENP-H (G-9): sc-365222 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Randy McDonald
Thank you for your question. Yes, CENP-H (G-9): sc-365222 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from Superb immunoperoxidase punctated nuclear Superb immunoperoxidase punctated nuclear and cytoplasmic staining in formalin fixed, paraffin-embedded human small intestine tissue. -SCBT QC
Date published: 2015-03-27
Rated 5 out of 5 by from Nice Western Blot data of CENP Nice Western Blot data of CENP-H expression in non-transfected 293T, human CENP-H transfected 293T and Jurkat whole cell lysates. -SCBT QC
Date published: 2013-02-02
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