CENP-F CRISPR/Cas9 KO Plasmid (h): sc-402178
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CENP-F CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CENP-F genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CENP-F CRISPR/Cas9 KO Plasmid (h) | sc-402178 | 20 µg | $397.00 |
Overview
CENPF encodes CENP-F, a large kinetochore-associated protein that accumulates in late G2 and mitosis and coordinates chromosome alignment, spindle checkpoint signaling, and faithful segregation during cell division. CENP-F contributes to microtubule attachment at kinetochores and supports centrosome function and spindle dynamics, linking it to core mitotic progression and genome stability pathways. Dysregulation of CENPF expression or localization is associated with chromosomal instability and altered proliferative capacity, features frequently investigated in cancer biology. CENP-F also serves as a marker of cell-cycle state and is studied for its roles in nuclear architecture and mitotic stress responses in human cells.
CENP-F CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CENPF gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CENPF together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CENPF open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CENP-F protein expression.
This CRISPR knockout system enables efficient generation of CENPF-deficient cell models for investigation of CENP-F signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CENPF exon(s) critical for CENP-F function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CENPF genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CENP-F CRISPR/Cas9 KO Plasmid (h) and CENP-F CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CENPF locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CENP-F HDR Plasmid (h) and CENP-F HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CENPF homology arms to support homology-directed repair at defined CENPF target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.