CENP-B Antibody (C-5): sc-365474
- CENP-B Antibody (C-5) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 579-599 at the C-terminus of CENP-B of human origin
- recommended for detection of CENP-B of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and equine, canine, bovine and porcine
- m-IgG1 BP-HRP is the preferred secondary detection reagent for CENP-B Antibody (C-5) for WB applications. This reagent is now offered in a bundle with CENP-B Antibody (C-5) (see ordering information below).
CENP-B Antibody (C-5) is a mouse monoclonal IgG1 antibody that detects CENP-B in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CENP-B plays a crucial role in the structure and function of centromeres, which are essential for accurate chromosome segregation during cell division. CENP-B is primarily located in the centromeric heterochromatin, where CENP-B interacts with DNA satellites to promote and maintain the cohesion of sister chromatids. This localization is vital as CENP-B ensures the integrity of the centromere, protecting it from nucleolytic degradation and maintaining its morphology, which is critical for proper chromosome alignment and segregation. Additionally, CENP-B′s ability to target centromeric a-DNA highlights CENP-B′s importance in safeguarding the genetic material during mitosis, making CENP-B a key player in cellular division and stability. anti-CENP-B antibody (C-5) is an invaluable tool for researchers studying the dynamics of centromere function and its implications in cell cycle regulation and chromosomal disorders.
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CENP-B Antibody (C-5) References:
- CENP-B box is required for de novo centromere chromatin assembly on human alphoid DNA. | Ohzeki, J., et al. 2002. J Cell Biol. 159: 765-75. PMID: 12460987
- CENP-B interacts with CENP-C domains containing Mif2 regions responsible for centromere localization. | Suzuki, N., et al. 2004. J Biol Chem. 279: 5934-46. PMID: 14612452
- The role of CENP-B and alpha-satellite DNA: de novo assembly and epigenetic maintenance of human centromeres. | Masumoto, H., et al. 2004. Chromosome Res. 12: 543-56. PMID: 15289662
- Identification of a subdomain of CENP-B that is necessary and sufficient for localization to the human centromere. | Pluta, AF., et al. 1992. J Cell Biol. 116: 1081-93. PMID: 1740467
- CENP-B controls centromere formation depending on the chromatin context. | Okada, T., et al. 2007. Cell. 131: 1287-300. PMID: 18160038
- CENP-B is a highly conserved mammalian centromere protein with homology to the helix-loop-helix family of proteins. | Sullivan, KF. and Glass, CA. 1991. Chromosoma. 100: 360-70. PMID: 1893793
- CENP-B preserves genome integrity at replication forks paused by retrotransposon LTR. | Zaratiegui, M., et al. 2011. Nature. 469: 112-5. PMID: 21151105
- Visualization of centromere proteins CENP-B and CENP-C on a stable dicentric chromosome in cytological spreads. | Earnshaw, WC., et al. 1989. Chromosoma. 98: 1-12. PMID: 2475307
- Proteomics characterization of CENP-B epitope in Moroccan scleroderma patients with anti-centromere autoantibodies. | Zian, Z., et al. 2020. Immunol Lett. 221: 1-5. PMID: 32057908
- Cytotoxic effect of combining two antisense oligonucleotides against telomerase rna component (hTR and mRNA of centromere protein B (CENP-B) in hepatocellular carcinoma cells. | El-Desoky, AM., et al. 2022. An Acad Bras Cienc. 94: e20200573. PMID: 35894385
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CENP-B Antibody (C-5) | sc-365474 | 200 µg/ml | $322.00 | |||
CENP-B Antibody (C-5): m-IgG1 BP-HRP Bundle | sc-542690 | 200 µg Ab; 20 µg BP | $361.00 | |||
CENP-B (C-5) Neutralizing Peptide | sc-365474 P | 100 µg/0.5 ml | $69.00 |