CEM15 Antibody (B-2): sc-390254
- CEM15 Antibody (B-2) is a mouse monoclonal IgG1 κ CEM15 antibody provided at 200 µg/ml
- raised against amino acids 241-310 mapping within an internal region of CEM15 of mouse origin
- CEM15 Antibody (B-2) is recommended for detection of CEM15 of mouse, rat origin by WB, IP, IF and ELISA
- Anti-CEM15 Antibody (B-2) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CEM15 Antibody (B-2) for WB applications. These reagents are now offered in bundles with CEM15 Antibody (B-2) (see ordering information below).
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CEM15 Antibody (B-2) is a mouse monoclonal IgG1 kappa light chain antibody that detects CEM15 protein of mouse and rat origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CEM15 (B-2) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CEM15, also known as APOBEC3G, plays a crucial role in the innate immune response against retroviruses, particularly HIV-1. APOBEC3G functions by deaminating deoxycytosine to deoxyuracil in the minus strand of HIV-1 DNA, leading to G to A hypermutation in the plus strand, which significantly impairs viral replication. This mutagenic activity is vital for controlling viral infections and contributes to the genetic diversity observed in many viruses. Notably, the interaction between APOBEC3G and the viral infectivity factor (Vif) is critical; Vif counteracts the antiviral effects of APOBEC3G by promoting its degradation, thus allowing HIV-1 to evade the host′s immune response. Mouse APOBEC3G remains resistant to Vif, making APOBEC3G a potent inhibitor of wild-type HIV-1 replication, unlike its human counterpart, which is only effective against Vif-deficient strains. This unique characteristic of mouse APOBEC3G highlights its potential as a target for therapeutic strategies aimed at enhancing antiviral responses. APOBEC3G is composed of 429 amino acids and serves as an important model for understanding the mechanisms of viral resistance and the development of antiviral therapies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CEM15 Antibody (B-2) References:
- The cytidine deaminase CEM15 induces hypermutation in newly synthesized HIV-1 DNA. | Zhang, H., et al. 2003. Nature. 424: 94-8. PMID: 12808465
- Broad antiretroviral defence by human APOBEC3G through lethal editing of nascent reverse transcripts. | Mangeat, B., et al. 2003. Nature. 424: 99-103. PMID: 12808466
- DNA deamination mediates innate immunity to retroviral infection. | Harris, RS., et al. 2003. Cell. 113: 803-9. PMID: 12809610
- Species-specific exclusion of APOBEC3G from HIV-1 virions by Vif. | Mariani, R., et al. 2003. Cell. 114: 21-31. PMID: 12859895
- The enzymatic activity of CEM15/Apobec-3G is essential for the regulation of the infectivity of HIV-1 virion but not a sole determinant of its antiviral activity. | Shindo, K., et al. 2003. J Biol Chem. 278: 44412-6. PMID: 12970355
- HIV-1 Vif blocks the antiviral activity of APOBEC3G by impairing both its translation and intracellular stability. | Stopak, K., et al. 2003. Mol Cell. 12: 591-601. PMID: 14527406
- The human immunodeficiency virus type 1 Vif protein reduces intracellular expression and inhibits packaging of APOBEC3G (CEM15), a cellular inhibitor of virus infectivity. | Kao, S., et al. 2003. J Virol. 77: 11398-407. PMID: 14557625
- Exhaustive genotyping of the CEM15 (APOBEC3G) gene and absence of association with AIDS progression in a French cohort. | Do, H., et al. 2005. J Infect Dis. 191: 159-63. PMID: 15609224
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CEM15 Antibody (B-2) | sc-390254 | 200 µg/ml | $322.00 | |||
CEM15 Antibody (B-2): m-IgG Fc BP-HRP Bundle | sc-530106 | 200 µg Ab; 10 µg BP | $361.00 | |||
CEM15 Antibody (B-2): m-IgGκ BP-HRP Bundle | sc-523465 | 200 µg Ab, 40 µg BP | $361.00 | |||
CEM15 Antibody (B-2): m-IgG1 BP-HRP Bundle | sc-543872 | 200 µg Ab; 20 µg BP | $361.00 | |||
CEM15 Antibody (B-2) AC | sc-390254 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CEM15 Antibody (B-2) HRP | sc-390254 HRP | 200 µg/ml | $322.00 | |||
CEM15 Antibody (B-2) FITC | sc-390254 FITC | 200 µg/ml | $336.00 | |||
CEM15 Antibody (B-2) PE | sc-390254 PE | 200 µg/ml | $349.00 | |||
CEM15 Antibody (B-2) Alexa Fluor® 488 | sc-390254 AF488 | 200 µg/ml | $364.00 | |||
CEM15 Antibody (B-2) Alexa Fluor® 546 | sc-390254 AF546 | 200 µg/ml | $364.00 | |||
CEM15 Antibody (B-2) Alexa Fluor® 594 | sc-390254 AF594 | 200 µg/ml | $364.00 | |||
CEM15 Antibody (B-2) Alexa Fluor® 647 | sc-390254 AF647 | 200 µg/ml | $364.00 | |||
CEM15 Antibody (B-2) Alexa Fluor® 680 | sc-390254 AF680 | 200 µg/ml | $364.00 | |||
CEM15 Antibody (B-2) Alexa Fluor® 790 | sc-390254 AF790 | 200 µg/ml | $364.00 |