Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CEL Double Nickase Plasmid (h): sc-403176-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CEL Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CEL Double Nickase Plasmid (h) and CEL Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CEL. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CEL Antibody (E-4): sc-377087
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CEL Double Nickase Plasmid (h) | sc-403176-NIC | 20 µg | $410.00 | |||
CEL Double Nickase Plasmid (h2) | sc-403176-NIC-2 | 20 µg | $410.00 |
Human CEL encodes carboxyl ester lipase, a secreted glycoenzyme best known for hydrolyzing dietary cholesteryl esters and triglycerides and contributing to intestinal lipid absorption. CEL activity intersects with lipid handling pathways by shaping the pool of free cholesterol, fatty acids, and monoacylglycerols available for lipoprotein assembly and downstream metabolic signaling. In the exocrine pancreas, CEL is part of the digestive enzyme milieu and can influence the composition of lipid-derived ligands that modulate inflammatory and metabolic responses. Altered CEL function or expression has been associated with dysregulated lipid metabolism and pancreatic or metabolic phenotypes, making it a relevant target for mechanistic studies of lipid digestion, secretion biology, and disease-associated metabolic stress.
CEL Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CEL locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CEL. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CEL function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CEL-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.