Cdk8 CRISPR/Cas9 KO Plasmid (m): sc-435217
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cdk8 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Cdk8 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cdk8 Antibody (D-9): sc-13155
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdk8 CRISPR/Cas9 KO Plasmid (m) | sc-435217 | 20 µg | $397.00 |
Overview
Cyclin-dependent kinase 8 (Cdk8) is a Mediator complex–associated serine/threonine kinase that regulates RNA polymerase II–dependent transcription by phosphorylating transcription factors and components of the transcriptional machinery. In mouse cells, Cdk8 integrates signals from pathways such as Wnt/β-catenin, TGF-β/SMAD, and MAPK to modulate cell-cycle progression, differentiation programs, and stress-responsive gene expression. Through its control of enhancer and promoter activity, Cdk8 influences metabolic and inflammatory transcriptional outputs and can shape lineage-specific transcriptional networks. Dysregulated CDK8 activity has been linked to aberrant transcriptional regulation in oncogenic and developmental contexts, making Cdk8 a relevant target for mechanistic studies of transcriptional control and signaling cross-talk.
Cdk8 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cdk8 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cdk8 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cdk8 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Cdk8 protein expression.
This CRISPR knockout system enables efficient generation of Cdk8-deficient cell models for investigation of Cdk8 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cdk8 exon(s) critical for Cdk8 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cdk8 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Cdk8 CRISPR/Cas9 KO Plasmid (m) and Cdk8 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cdk8 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Cdk8 HDR Plasmid (m) and Cdk8 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cdk8 homology arms to support homology-directed repair at defined Cdk8 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.