CDK5RAP2 Antibody (527CT22.9.2): sc-517321

CDK5RAP2 Antibody (527CT22.9.2) is a mouse monoclonal IgM antibody that detects CDK5RAP2 of human origin by western blotting (WB). Anti-CDK5RAP2 antibody (527CT22.9.2) is available as a non-conjugated format. CDK5RAP2, also known as Centrosome-associated protein 215, plays a crucial role in regulating cyclin-dependent kinase 5 (Cdk5) activity, a key player in neuronal differentiation and cell cycle progression. Through complex formation with CDK5RAP1, CDK5RAP2 inhibits Cdk5 activation by p35, thereby modulating kinase activity and ensuring proper neuronal function. CDK5RAP2 is predominantly expressed in various tissues, including placenta, liver, pancreas, heart, skeletal muscle, lung, brain, and kidney, and associates with centrosomes throughout the cell cycle, highlighting importance in cellular organization and division. Mutations in CDK5RAP2 are linked to primary microcephaly autosomal recessive type 3, a condition characterized by significantly reduced head size and neurological deficits, underscoring critical role in brain development. Four isoforms of CDK5RAP2 are produced through alternative splicing, which may contribute to diverse functional roles in cellular processes.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CDK5RAP2 Antibody (527CT22.9.2) References:

1. Molecular evolution of the brain size regulator genes CDK5RAP2 and CENPJ.  |  Evans, PD., et al. 2006. Gene. 375: 75-9. PMID: 16631324
2. Molecular genetic determinants of human brain size.  |  Tang, BL. 2006. Biochem Biophys Res Commun. 345: 911-6. PMID: 16716254
3. What primary microcephaly can tell us about brain growth.  |  Cox, J., et al. 2006. Trends Mol Med. 12: 358-66. PMID: 16829198
4. Transient response to imatinib in a chronic eosinophilic leukemia associated with ins(9;4)(q33;q12q25) and a CDK5RAP2-PDGFRA fusion gene.  |  Walz, C., et al. 2006. Genes Chromosomes Cancer. 45: 950-6. PMID: 16845659
5. CDK5RAP2 is a pericentriolar protein that functions in centrosomal attachment of the gamma-tubulin ring complex.  |  Fong, KW., et al. 2008. Mol Biol Cell. 19: 115-25. PMID: 17959831
6. Cep68 and Cep215 (Cdk5rap2) are required for centrosome cohesion.  |  Graser, S., et al. 2007. J Cell Sci. 120: 4321-31. PMID: 18042621
7. CDK5RAP2 is required for spindle checkpoint function.  |  Zhang, X., et al. 2009. Cell Cycle. 8: 1206-16. PMID: 19282672
8. Interaction of CDK5RAP2 with EB1 to track growing microtubule tips and to regulate microtubule dynamics.  |  Fong, KW., et al. 2009. Mol Biol Cell. 20: 3660-70. PMID: 19553473
9. Cdk5rap2 exposes the centrosomal root of microcephaly syndromes.  |  Megraw, TL., et al. 2011. Trends Cell Biol. 21: 470-80. PMID: 21632253
10. Congenital microcephaly-linked CDK5RAP2 affects eye development.  |  Zaqout, S., et al. 2020. Ann Hum Genet. 84: 87-91. PMID: 31355417
11. CDK5RAP2 loss-of-function causes premature cell senescence via the GSK3β/β-catenin-WIP1 pathway.  |  Wang, X., et al. 2021. Cell Death Dis. 13: 9. PMID: 34930892
12. CDK5RAP2 is a Wnt target gene and promotes stemness and progression of oral squamous cell carcinoma.  |  Shen, Y., et al. 2023. Cell Death Dis. 14: 107. PMID: 36774351