CDK5RAP1 Double Nickase Plasmid (h): sc-407278-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CDK5RAP1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CDK5RAP1 Double Nickase Plasmid (h) and CDK5RAP1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CDK5RAP1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CDK5RAP1 Antibody (D-1): sc-398764
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CDK5RAP1 Double Nickase Plasmid (h) | sc-407278-NIC | 20 µg | $410.00 | |||
CDK5RAP1 Double Nickase Plasmid (h2) | sc-407278-NIC-2 | 20 µg | $410.00 |
CDK5RAP1 (CDK5 regulatory subunit–associated protein 1) is a mitochondrial enzyme implicated in post-transcriptional regulation through tRNA modification, supporting efficient mitochondrial translation and oxidative phosphorylation. By modulating the integrity of mitochondrial protein synthesis, CDK5RAP1 influences cellular bioenergetics, redox balance, and stress adaptation pathways. Perturbation of CDK5RAP1 function has been linked to altered mitochondrial homeostasis and is studied in contexts where metabolic remodeling and mitochondrial dysfunction contribute to disease biology, including cancer and neurodegeneration. As a mitochondria-associated regulator, CDK5RAP1 provides a tractable node for dissecting how mitochondrial gene expression impacts cell state and survival under stress.
CDK5RAP1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CDK5RAP1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CDK5RAP1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CDK5RAP1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CDK5RAP1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.