Cdk4 Antibody (3F121): sc-70831

Cdk4 Antibody (3F121) is a mouse monoclonal IgG1 kappa light chain antibody designed to detect Cyclin-dependent kinase 4 (Cdk4), also known as CDC2-like kinase 4, across mouse, rat, and human species. anti-Cdk4 antibody (3F121) targets the full-length recombinant human Cdk4 protein, making Cdk4 (3F121) antibody suitable for applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry with paraffin-embedded sections (IHCP). Cdk4 is a crucial serine/threonine kinase involved in regulating the cell cycle, particularly the transition from the G1 to the S phase, by forming complexes with D-type cyclins. The structure of Cdk4, which includes kinase domain and cyclin-binding regions, is essential for phosphorylating key substrates such as the retinoblastoma protein (Rb), thereby driving cellular proliferation and ensuring proper cell cycle progression. Post-translational modifications of Cdk4, particularly phosphorylation, are vital for activation and regulation, enabling precise control over cell division and preventing uncontrolled proliferation that can lead to cancer. Additionally, Cdk4 interacts with regulatory proteins like the inhibitor p16, which modulates activity to maintain cellular homeostasis. Alterations in Cdk4 structure or regulatory mechanisms are frequently associated with various cancers, underscoring significance as a target for therapeutic interventions. Available in both non-conjugated and various conjugated forms, including horseradish peroxidase (HRP) and fluorescent tags, Cdk4 (3F121) monoclonal antibody provides flexibility to meet diverse experimental requirements. By facilitating detailed analysis of Cdk4′s role and regulation within the cell, anti-Cdk4 antibody (3F121) supports advancements in cancer research and the development of targeted therapies.

The preferred Cdk4 antibody is Cdk4 Antibody (DCS-35): sc-23896.