Cdk2 Antibody (D-12): sc-6248

Cdk2 Antibody (D-12) is a mouse monoclonal IgG1 kappa light chain antibody specifically targeting Cyclin-dependent kinase 2 (Cdk2). Cdk2 monoclonal antibody (D-12) is raised against amino acids 1-298, representing the full-length human Cdk2 protein, and effectively recognizes Cdk2 from mouse, rat, and human species in applications such as Western Blot (WB), Immunoprecipitation (IP), Immunofluorescence (IF), and ELISA. Cdk2 plays a crucial role in cell cycle regulation by forming complexes with cyclins E and A, which are essential for the transition from the G1 to S phase and the progression through the S phase, respectively. This kinase′s activity ensures proper DNA replication and cell division, making Cdk2 a key target for studying cell proliferation and cancer mechanisms. Cdk2 monoclonal antibody (D-12) is available in both non-conjugated form and various conjugated forms, including agarose, HRP, PE, FITC, and multiple Alexa Fluor® conjugates, providing versatility for different experimental needs. By facilitating the detection and analysis of Cdk2, Cdk2 (D-12) antibody supports research into cell cycle dynamics, cancer biology, and the development of therapeutic interventions targeting cell proliferation.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Cdk2 Antibody (D-12) References:

1. Cyclin A is required at two points in the human cell cycle.  |  Pagano, M., et al. 1992. EMBO J. 11: 961-71. PMID: 1312467
2. CDK2 encodes a 33-kDa cyclin A-associated protein kinase and is expressed before CDC2 in the cell cycle.  |  Elledge, SJ., et al. 1992. Proc Natl Acad Sci U S A. 89: 2907-11. PMID: 1372993
3. Distinct forms of human CDC2 identified by novel monoclonal antibodies.  |  Lukás, J., et al. 1992. Eur J Biochem. 207: 169-76. PMID: 1628647
4. Human D-type cyclin.  |  Xiong, Y., et al. 1991. Cell. 65: 691-9. PMID: 1827756
5. Cyclins and their partners during Xenopus oocyte maturation.  |  Kobayashi, H., et al. 1991. Cold Spring Harb Symp Quant Biol. 56: 437-47. PMID: 1840257
6. Reversible tyrosine phosphorylation of cdc2: dephosphorylation accompanies activation during entry into mitosis.  |  Morla, AO., et al. 1989. Cell. 58: 193-203. PMID: 2473839
7. The cdc2 kinase is a nuclear protein that is essential for mitosis in mammalian cells.  |  Riabowol, K., et al. 1989. Cell. 57: 393-401. PMID: 2541912
8. Isolation of a human cyclin cDNA: evidence for cyclin mRNA and protein regulation in the cell cycle and for interaction with p34cdc2.  |  Pines, J. and Hunter, T. 1989. Cell. 58: 833-46. PMID: 2570636
9. Cip1 blocks the initiation of DNA replication in Xenopus extracts by inhibition of cyclin-dependent kinases.  |  Strausfeld, UP., et al. 1994. Curr Biol. 4: 876-83. PMID: 7850420
10. G2 delay induced by nitrogen mustard in human cells affects cyclin A/cdk2 and cyclin B1/cdc2-kinase complexes differently.  |  O'Connor, PM., et al. 1993. J Biol Chem. 268: 8298-308. PMID: 8463339
11. Expression and subcellular localization of CDK2 and cdc2 kinases and their common partner cyclin A in thyroid epithelial cells: comparison of cyclic AMP-dependent and -independent cell cycles.  |  Baptist, M., et al. 1996. J Cell Physiol. 166: 256-73. PMID: 8591985
12. Both cyclin A and cyclin E have S-phase promoting (SPF) activity in Xenopus egg extracts.  |  Strausfeld, UP., et al. 1996. J Cell Sci. 109 (Pt 6): 1555-63. PMID: 8799842

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cdk2 Antibody (D-12) has 494 citations in a variety of scientific publications.

Primary antibodies like Anti-Cdk2 Antibody (D-12) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cdk2 Antibody (D-12) Specifications

Alternate Product Name: Cdk2

Product Clone Name: D-12

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-298

Epitope Target: Cdk2

Epitope Species: human

Product Reactivity/Detection: Cdk2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 494 citations in various scientific publications

Available Conjugates/Forms for Cdk2 Antibody (D-12): AC: Agarose, AF405: Alexa Fluor^® 405, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, TRITC: Rhodamine