Cdk1/Cdk2 Antibody (AN21.2): sc-53219

Cdk1/Cdk2 Antibody (AN21.2) is a mouse monoclonal IgG2a kappa light chain antibody that detects Cdk1/Cdk2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and immunohistochemistry. anti-Cdk1/Cdk2 antibody (AN21.2) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cdk1 and Cdk2 proteins play crucial roles in cell cycle regulation, particularly in the transition from the G2 phase to mitosis. These cyclin-dependent kinases are essential for proper progression of the cell cycle, as they phosphorylate target proteins that drive cells through various checkpoints. Notably, Cdk1 is primarily active during the G2/M transition, while Cdk2 is involved in the G1/S transition, highlighting their distinct yet complementary functions in cell cycle control. Dysregulation of these kinases can lead to uncontrolled cell proliferation, a hallmark of cancer, making them important targets for therapeutic intervention. Cdk1/Cdk2 (AN21.2) monoclonal antibody is therefore a valuable tool for researchers studying cell cycle dynamics and molecular mechanisms underlying cell division.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

Cdk1/Cdk2 Antibody (AN21.2) References:

1. Cyclin A is required at two points in the human cell cycle.  |  Pagano, M., et al. 1992. EMBO J. 11: 961-71. PMID: 1312467
2. CDK2 encodes a 33-kDa cyclin A-associated protein kinase and is expressed before CDC2 in the cell cycle.  |  Elledge, SJ., et al. 1992. Proc Natl Acad Sci U S A. 89: 2907-11. PMID: 1372993
3. Human D-type cyclin.  |  Xiong, Y., et al. 1991. Cell. 65: 691-9. PMID: 1827756
4. The cdc2 kinase is a nuclear protein that is essential for mitosis in mammalian cells.  |  Riabowol, K., et al. 1989. Cell. 57: 393-401. PMID: 2541912
5. Isolation of a human cyclin cDNA: evidence for cyclin mRNA and protein regulation in the cell cycle and for interaction with p34cdc2.  |  Pines, J. and Hunter, T. 1989. Cell. 58: 833-46. PMID: 2570636
6. Cip1 blocks the initiation of DNA replication in Xenopus extracts by inhibition of cyclin-dependent kinases.  |  Strausfeld, UP., et al. 1994. Curr Biol. 4: 876-83. PMID: 7850420
7. Expression and subcellular localization of CDK2 and cdc2 kinases and their common partner cyclin A in thyroid epithelial cells: comparison of cyclic AMP-dependent and -independent cell cycles.  |  Baptist, M., et al. 1996. J Cell Physiol. 166: 256-73. PMID: 8591985
8. Both cyclin A and cyclin E have S-phase promoting (SPF) activity in Xenopus egg extracts.  |  Strausfeld, UP., et al. 1996. J Cell Sci. 109 (Pt 6): 1555-63. PMID: 8799842
9. Cell cycle regulatory proteins--an overview with relevance to oral cancer.  |  Goodger, NM., et al. 1997. Oral Oncol. 33: 61-73. PMID: 9231162

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cdk1/Cdk2 Antibody (AN21.2) has 74 citations in a variety of scientific publications.

Primary antibodies like Anti-Cdk1/Cdk2 Antibody (AN21.2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cdk1/Cdk2 Antibody (AN21.2) Specifications

Alternate Product Name: Cdk1/Cdk2

Product Clone Name: AN21.2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: Cdk2

Epitope Species: human

Product Reactivity/Detection: Cdk1/Cdk2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P)

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 74 citations in various scientific publications

Available Conjugates/Forms for Cdk1/Cdk2 Antibody (AN21.2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin