CDCP1 CRISPR/Cas9 KO Plasmid (m): sc-430933
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CDCP1 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CDCP1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CDCP1 CRISPR/Cas9 KO Plasmid (m) | sc-430933 | 20 µg | $397.00 |
Overview
Cdcp1 encodes CDCP1, a single-pass transmembrane glycoprotein enriched at the cell surface where it functions as a scaffolding and signaling hub for adhesion- and stress-responsive pathways. CDCP1 undergoes tyrosine phosphorylation and engages Src family kinases and PKC signaling, influencing cytoskeletal remodeling, cell migration, survival, and extracellular matrix interactions. In mouse systems, Cdcp1 activity has been linked to epithelial and stromal cell behavior, anoikis resistance, and context-dependent regulation of invasive phenotypes. Dysregulated CDCP1 signaling is frequently studied in models of tumor progression, metastasis-associated programs, and inflammatory microenvironments, making it relevant for mechanistic pathway interrogation.
CDCP1 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Cdcp1 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Cdcp1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Cdcp1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CDCP1 protein expression.
This CRISPR knockout system enables efficient generation of Cdcp1-deficient cell models for investigation of CDCP1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Cdcp1 exon(s) critical for CDCP1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Cdcp1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CDCP1 CRISPR/Cas9 KO Plasmid (m) and CDCP1 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Cdcp1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CDCP1 HDR Plasmid (m) and CDCP1 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Cdcp1 homology arms to support homology-directed repair at defined Cdcp1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.