Cdc7 Antibody (DCS-341): sc-56274
- Cdc7 Antibody (DCS-341) is a mouse monoclonal IgG1 κ, cited in 3 publications, provided at 200 µg/ml
- raised against full length Cdc7 of human origin
- recommended for detection of Cdc7 of human origin by WB, IP, IF and ELISA
- m-IgG Fc BP-HRP is the preferred secondary detection reagent for Cdc7 Antibody (DCS-341) for WB applications. This reagent is now offered in a bundle with Cdc7 Antibody (DCS-341) (see ordering information below).
Cdc7 Antibody (DCS-341) is a mouse monoclonal IgG1 antibody that detects Cdc7 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Cdc7 is a crucial protein kinase that plays a vital role in the activation of replication origins during the S phase of the cell cycle, ensuring proper initiation of DNA replication. The Cdc7/Dbf4 complex, consisting of the catalytic subunit Cdc7 and regulatory subunit Dbf4, is responsible for phosphorylating key proteins necessary for DNA replication, including several minichromosome maintenance (Mcm) proteins and the p180 subunit of DNA polymerase α-primase. Regulation of Cdc7 activity is tightly controlled throughout the cell cycle, with Dbf4 levels fluctuating significantly; Dbf4 is absent during the G1 phase but accumulates during the S and G2 phases, which is critical for the timing of DNA replication. The anaphase-promoting complex further modulates Dbf4 stability by promoting degradation during chromosome segregation, preventing premature reactivation of prereplicative complexes. This precise regulation is essential for maintaining genomic integrity and ensuring that DNA replication occurs only once per cell cycle, highlighting the importance of Cdc7 in cellular proliferation and potential implications of dysregulation in cancer and other diseases.
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Cdc7 Antibody (DCS-341) References:
- CDC7 kinase complex as a molecular switch for DNA replication. | Masai, H., et al. 1999. Front Biosci. 4: D834-40. PMID: 10577390
- Functions of mammalian Cdc7 kinase in initiation/monitoring of DNA replication and development. | Kim, JM., et al. 2003. Mutat Res. 532: 29-40. PMID: 14643427
- Regulation of chromosome dynamics by Hsk1/Cdc7 kinase. | Matsumoto, S. and Masai, H. 2013. Biochem Soc Trans. 41: 1712-9. PMID: 24256280
- Targeting CDC7 improves sensitivity to chemotherapy of esophageal squamous cell carcinoma. | Cao, JX. and Lu, Y. 2019. Onco Targets Ther. 12: 63-74. PMID: 30588031
- Cdc7-mediated phosphorylation of Cse4 regulates high-fidelity chromosome segregation in budding yeast. | Mishra, PK., et al. 2021. Mol Biol Cell. 32: ar15. PMID: 34432494
- Targeting CDC7 potentiates ATR-CHK1 signaling inhibition through induction of DNA replication stress in liver cancer. | Guo, Y., et al. 2021. Genome Med. 13: 166. PMID: 34663432
- CDC7 kinase (DDK) inhibition disrupts DNA replication leading to mitotic catastrophe in Ewing sarcoma. | Martin, JC., et al. 2022. Cell Death Discov. 8: 85. PMID: 35220396
- The structural basis of Cdc7-Dbf4 kinase dependent targeting and phosphorylation of the MCM2-7 double hexamer. | Saleh, A., et al. 2022. Nat Commun. 13: 2915. PMID: 35614055
- Cancer-associated FBXW7 loss is synthetic lethal with pharmacological targeting of CDC7. | Baxter, JS., et al. 2024. Mol Oncol. 18: 369-385. PMID: 37866880
- Protein kinase Cdc7 supports viral replication by phosphorylating Avibirnavirus VP3 protein. | Deng, T., et al. 2023. J Virol. 97: e0112523. PMID: 37902398
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdc7 Antibody (DCS-341) | sc-56274 | 200 µg/ml | $322.00 | |||
Cdc7 Antibody (DCS-341): m-IgG Fc BP-HRP Bundle | sc-551936 | 200 µg Ab; 10 µg BP | $361.00 |