Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Cdc6 Double Nickase Plasmid (h): sc-400796-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cdc6 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Cdc6 Double Nickase Plasmid (h) and Cdc6 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CDC6. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cdc6 Antibody (180.2): sc-9964
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdc6 Double Nickase Plasmid (h) | sc-400796-NIC | 20 µg | $410.00 | |||
Cdc6 Double Nickase Plasmid (h2) | sc-400796-NIC-2 | 20 µg | $410.00 |
CDC6 encodes the human Cdc6 ATPase, a core component of the pre-replication complex that cooperates with ORC and CDT1 to load the MCM2–7 helicase at replication origins, licensing DNA synthesis during G1 phase. Cdc6 activity is coordinated with CDK-dependent control and replication checkpoint signaling to ensure once-per-cell-cycle genome duplication, linking replication origin firing to cell-cycle progression and DNA damage responses. Dysregulated CDC6 expression or function can promote replication stress, genomic instability, and aberrant S-phase entry, processes frequently studied in proliferative disorders and cancer biology. As a replication licensing factor, Cdc6 is also relevant to investigations of chromatin dynamics, origin selection, and mechanisms that suppress re-replication.
Cdc6 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CDC6 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CDC6. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CDC6 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CDC6-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.