Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CDC42 Double Nickase Plasmid (h): sc-418353-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CDC42 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CDC42 Double Nickase Plasmid (h) and CDC42 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CDC42. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cdc42 Antibody (B-8): sc-8401
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CDC42 Double Nickase Plasmid (h) | sc-418353-NIC | 20 µg | $410.00 | |||
CDC42 Double Nickase Plasmid (h2) | sc-418353-NIC-2 | 20 µg | $410.00 |
CDC42 encodes a ubiquitously expressed Rho family small GTPase that functions as a molecular switch controlling actin cytoskeleton remodeling, cell polarity, membrane trafficking, and cytokinesis. By cycling between GTP- and GDP-bound states under the control of GEFs, GAPs, and GDIs, CDC42 coordinates signaling through effectors such as PAKs, WASP/N-WASP, and the PAR polarity complex to regulate adhesion dynamics and directional migration. It intersects with pathways governing endocytosis, vesicle budding, and mitotic spindle orientation, linking cytoskeletal architecture to cell-cycle progression. Dysregulated CDC42 signaling has been associated with altered proliferation and invasion phenotypes in cancer biology and with neurodevelopmental and immune-related disorders where polarity and trafficking are perturbed.
CDC42 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CDC42 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CDC42. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CDC42 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CDC42-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.