Every item is shipped based on the best shipping method assessed for the temperature requirements of that specific item. Items are grouped and shipped together whenever
possible, and a separate shipping charge will be included for each shipping method required. Shipping charges listed below are from our US warehouses to the Contiguous US,
Alaska, Hawaii, Canada and Puerto Rico. Shipping charges for countries outside the US and Canada will be determined once order has been received
Please note: We can not ship to PO boxes
Express Blue Ice
Express Dry Ice
Animal Health Prescription Item
SHIPPING METHODS & CHARGES
Ships via FedEx Ground to Contiguous US, Alaska, Canada, Monday through Friday. This method is used for less temperature sensitive items such as lab ware and animal
health products, bulky and/or heavy items
Labware ships FedEx Ground free of charge to the contiguous US
The eukaryotic cell division cycle consists of a number of gene-controlled sequences that involve cyclin dependent kinases (Cdks) and cell division control (Cdc) proteins. Cdc34B, also known as UBE2R2 (Ubiquitin-conjugating enzyme E2 R2) or UBC3B, is a 238 amino acid member of the E2 ubiquitin-conjugating enzyme family. Similar to Cdc34, Cdc34B functions to catalytically attach ubiquitin to various proteins, such as β-TrCP (an F-box protein that mediates β-catenin degradation), via an ATP-dependent reaction that yields AMP, a diphosphate and a ubiquitin-tagged protein. Cdc34B can be phosphorylated by the protein kinase CK2 (Casein kinase II), thereby allowing Cdc34B to regulate β-TrCP substrate recognition and, ultimately, enhance β-catenin degradation. Due to its ability to control β-TrCP activity, Cdc34B is thought to play a key role in cell cycle progression.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
What is the recommended fixation method for immunofluorescence staining with Cdc34B (C-4): sc-376427 monoclonal antibody?
Asked by: jerojero
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: