Cdc34 Double Nickase Plasmid (h): sc-403783-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cdc34 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Cdc34 Double Nickase Plasmid (h) and Cdc34 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CDC34. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cdc34 Antibody (H-4): sc-28381
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdc34 Double Nickase Plasmid (h) | sc-403783-NIC | 20 µg | $410.00 | |||
Cdc34 Double Nickase Plasmid (h2) | sc-403783-NIC-2 | 20 µg | $410.00 |
Human CDC34 encodes Cdc34, an E2 ubiquitin-conjugating enzyme that partners with SCF (SKP1–CUL1–F-box) E3 ligases to assemble K48-linked polyubiquitin chains and promote proteasomal turnover of key regulators of the cell cycle and DNA replication. By controlling ubiquitination of substrates such as CDK inhibitors and replication licensing factors, Cdc34 helps coordinate G1/S progression, checkpoint signaling, and genome stability. Dysregulated CDC34 activity or expression has been linked to altered proteostasis and aberrant proliferation programs observed across multiple cancer-relevant contexts, making it a useful node for studying ubiquitin pathway dependencies. CDC34 is also leveraged to interrogate cullin-RING ligase circuitry, ubiquitin signaling dynamics, and stress-adaptive responses to proteotoxic or replicative stress.
Cdc34 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CDC34 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CDC34. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CDC34 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CDC34-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.