Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Cdc25A CRISPR/Cas9 KO Plasmid (h): sc-400345
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cdc25A CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the Cdc25A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cdc25A Antibody (F-6): sc-7389
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdc25A CRISPR/Cas9 KO Plasmid (h) | sc-400345 | 20 µg | $397.00 |
Overview
CDC25A encodes the human dual-specificity phosphatase Cdc25A, a key activator of cyclin-dependent kinases that promotes cell-cycle progression by dephosphorylating inhibitory CDK residues at the G1/S transition and during S-phase entry. Its activity is tightly controlled by checkpoint signaling, including ATR/ATM–CHK1/CHK2 pathways that regulate CDC25A stability in response to replication stress and DNA damage. Through coupling proliferative signaling to genome integrity surveillance, Cdc25A influences DNA replication timing, checkpoint recovery, and mitotic competence. Dysregulated CDC25A expression or turnover has been linked to aberrant proliferation and genomic instability, making it a common focus in studies of oncogenic signaling, checkpoint defects, and cell-cycle–driven disease mechanisms.
Cdc25A CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the CDC25A gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the CDC25A together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the CDC25A open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish Cdc25A protein expression.
This CRISPR knockout system enables efficient generation of CDC25A-deficient cell models for investigation of Cdc25A signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting CDC25A exon(s) critical for Cdc25A function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple CDC25A genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by Cdc25A CRISPR/Cas9 KO Plasmid (h) and Cdc25A CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the CDC25A locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by Cdc25A HDR Plasmid (h) and Cdc25A HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by CDC25A homology arms to support homology-directed repair at defined CDC25A target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.