Cdc2 p34 Double Nickase Plasmid (h): sc-400181-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cdc2 p34 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Cdc2 p34 Double Nickase Plasmid (h) and Cdc2 p34 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CDK1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cdc2 p34 Antibody (17): sc-54
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cdc2 p34 Double Nickase Plasmid (h) | sc-400181-NIC | 20 µg | $410.00 | |||
Cdc2 p34 Double Nickase Plasmid (h2) | sc-400181-NIC-2 | 20 µg | $410.00 |
CDK1 encodes the serine/threonine kinase Cdc2 p34, the core catalytic subunit of M-phase promoting factor that drives G2/M transition and mitotic progression. CDK1 activity is coordinated through cyclin binding, activating phosphorylation by CDK-activating kinase, and inhibitory control by WEE1/MYT1 and CDC25 phosphatases, linking checkpoint signaling to orderly chromosome segregation. As a central node in cell-cycle circuitry, CDK1 intersects DNA damage response and replication stress pathways to regulate mitotic entry, spindle assembly, and cytokinesis. Dysregulated CDK1 signaling and altered checkpoint control are frequently observed in proliferative disorders and provide a mechanistic handle for studying genome instability and cell-cycle dependence in human cell models.
Cdc2 p34 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CDK1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CDK1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CDK1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CDK1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.