Cdc2 p34 Antibody (B322) recommended for detection of Cdc2 p34 of mouse, rat and human origin by WB, IP, IF and IHC(P)

Cdc2 p34 Antibody (B322): sc-56261

Cdc2 p34 Antibody (B322) is rated 4.0 out of 5 by 3.
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Datasheets

    • Cdc2 p34 Antibody (B322) is a mouse monoclonal IgG2a provided at 100 µg/ml
    • raised against a synthetic peptide of human Cdc2 p34
    • recommended for detection of Cdc2 p34 of mouse, rat and human origin by WB, IP, IF and IHC(P); non cross-reactive with other related cyclin dependent kinases.
    • See Cdc2 p34 (17): sc-54 for Cdc2 p34 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
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Can sc-56261: Cdc2 p34 (B322) monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Germaine
Thank you for your question. Yes, sc-56261: Cdc2 p34 (B322) is recommended for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Endogenous expression in HeLa cells Endogenous expression in HeLa cells, strong band at correct size. - SCBT QC
Date published: 2015-04-17
Rated 4 out of 5 by from Performs well in Western Performs well in Western, good signal strength in non-transfected 293, human Cdc2 p34 transfected 293 and HeLa whole cell lysates. - SCBT QC
Date published: 2015-01-25
Rated 4 out of 5 by from Clean WB blot with single strong band in non Clean WB blot with single strong band in non-transfected 293T, mouse Cdc2 p34 transfected 293T and K-562 whole cell lysates. - SCBT QC
Date published: 2014-09-27
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