CD9P-1 CRISPR/Cas9 KO Plasmid (h): sc-404449
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD9P-1 CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CD9P-1 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD9P-1 CRISPR/Cas9 KO Plasmid (h) | sc-404449 | 20 µg | $397.00 |
Overview
PTGFRN encodes CD9P-1 (also known as EWI-F), a tetraspanin-associated immunoglobulin superfamily protein that organizes membrane microdomains through interactions with CD9/CD81 and links them to the actin cytoskeleton. CD9P-1 contributes to regulation of cell adhesion, migration, and signaling by coordinating integrin- and tetraspanin-enriched complexes at the plasma membrane. These processes intersect with pathways governing cytoskeletal remodeling, extracellular matrix engagement, and vesicle trafficking, which are frequently examined in studies of invasive behavior and tissue remodeling. Altered PTGFRN expression and CD9P-1 network perturbations have been reported in multiple disease-associated contexts, motivating mechanistic research in cancer cell biology and inflammation-related phenotypes.
CD9P-1 CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the PTGFRN gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the PTGFRN together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the PTGFRN open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CD9P-1 protein expression.
This CRISPR knockout system enables efficient generation of PTGFRN-deficient cell models for investigation of CD9P-1 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting PTGFRN exon(s) critical for CD9P-1 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple PTGFRN genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CD9P-1 CRISPR/Cas9 KO Plasmid (h) and CD9P-1 CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the PTGFRN locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CD9P-1 HDR Plasmid (h) and CD9P-1 HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by PTGFRN homology arms to support homology-directed repair at defined PTGFRN target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.