CD9 Double Nickase Plasmid (h): sc-400252-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD9 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD9 Double Nickase Plasmid (h) and CD9 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CD9. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD9 Antibody (C-4): sc-13118
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD9 Double Nickase Plasmid (h) | sc-400252-NIC | 20 µg | $410.00 | |||
CD9 Double Nickase Plasmid (h2) | sc-400252-NIC-2 | 20 µg | $410.00 |
CD9 is a tetraspanin cell-surface protein that organizes membrane microdomains by partnering with integrins, immunoglobulin superfamily receptors, and other tetraspanins to coordinate adhesion, motility, and membrane fusion events. In human cells, CD9 contributes to endocytosis and vesicle trafficking and modulates signaling outputs linked to cytoskeletal remodeling, including pathways influencing cell migration and extracellular matrix interactions. CD9 is also enriched on extracellular vesicles and is widely used as an exosome marker, supporting studies of intercellular communication and vesicle biogenesis. Altered CD9 expression and localization have been associated with dysregulated invasion, immune cell interactions, and metastatic behavior in multiple cancer contexts, making it relevant to mechanistic disease models.
CD9 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CD9 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CD9. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CD9 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CD9-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.