CD83 Antibody (F-5): sc-55535

CD83 Antibody (F-5) is a mouse monoclonal IgG2b kappa light chain antibody that detects CD83 of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). Anti-CD83 antibody (F-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD83 is a heavily glycosylated membrane protein belonging to the immunoglobulin superfamily, primarily expressed in mature dendritic cells, Langerhans cells, and interdigitating reticulum cells within lymphoid tissues. CD83 plays a crucial role in immune response regulation, particularly in the activation and maturation of dendritic cells, which are essential for initiating T cell responses against pathogens and tumors. Structurally, CD83 features an extracellular V-type immunoglobulin-like domain, a single transmembrane domain, and a 40-residue cytoplasmic tail, which are vital for cell signaling and adhesion. CD83 has been identified as a sialic acid-binding immunoglobulin-like lectin (Siglec) adhesion molecule, facilitating interactions between dendritic cells and monocytes as well as CD8+ T cells, thereby enhancing immune response. CD83 expression serves as a marker for mature antigen-presenting dendritic cells, which generate tumor-specific T cell immunity, highlighting potential as a target for anti-cancer vaccines.

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CD83 Antibody (F-5) References:

1. IL-18-induced CD83+CCR7+ NK helper cells.  |  Mailliard, RB., et al. 2005. J Exp Med. 202: 941-53. PMID: 16203865
2. CD83 polymorphisms and cervical cancer risk.  |  Yu, KJ., et al. 2009. Gynecol Oncol. 114: 319-22. PMID: 19446866
3. Involvement of Caveolin-1 in CD83 Internalization in Mouse Dendritic Cells.  |  Yang, Y., et al. 2015. Cell Transplant. 24: 1395-404. PMID: 24898475
4. Dendritic cell CD83 homotypic interactions regulate inflammation and promote mucosal homeostasis.  |  Bates, JM., et al. 2015. Mucosal Immunol. 8: 414-28. PMID: 25204675
5. Anti-CD83 promotes IgG1 isotype switch in marginal zone B cells in response to TI-2 antigen.  |  Kretschmer, B., et al. 2015. Immunobiology. 220: 964-75. PMID: 25766204
6. CD83 Antibody Inhibits Human B Cell Responses to Antigen as well as Dendritic Cell-Mediated CD4 T Cell Responses.  |  Wong, KY., et al. 2018. J Immunol. 200: 3383-3396. PMID: 29643191
7. CD83+ CCR7+ NK cells induced by interleukin 18 by dendritic cells promote experimental autoimmune uveitis.  |  Fu, Q., et al. 2019. J Cell Mol Med. 23: 1827-1839. PMID: 30548211
8. Soluble CD83 suppresses experimental food allergy via regulating aberrant T helper 2 responses.  |  Song, W., et al. 2020. Immunol Res. 68: 141-151. PMID: 32529460
9. Soluble CD83 inhibits acute rejection by up regulating TGF-β and IDO secretion in rat liver transplantation.  |  Xiong, L., et al. 2021. Transpl Immunol. 64: 101351. PMID: 33171217
10. Soluble CD83 Regulates Dendritic Cell-T Cell Immunological Synapse Formation by Disrupting Rab1a-Mediated F-Actin Rearrangement.  |  Lin, W., et al. 2020. Front Cell Dev Biol. 8: 605713. PMID: 33585445

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD83 Antibody (F-5) has 20 citations in a variety of scientific publications.

Primary antibodies like Anti-CD83 Antibody (F-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD83 Antibody (F-5) Specifications

Alternate Product Name: CD83

Product Clone Name: F-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2b kappa light chain

Amino Acids: 7-205

Epitope Target: CD83

Epitope Species: human

Product Reactivity/Detection: CD83

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), FCM, ELISA

Vialing Information: Each vial contains 200 µg IgG_2b kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 20 citations in various scientific publications

Available Conjugates/Forms for CD83 Antibody (F-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CD83 antibody is CD83 Antibody (HB15a): sc-19677.