CD8 Antibody (32-M4): sc-1177

CD8 Antibody (32-M4) is a mouse monoclonal IgG2a kappa light chain antibody that detects CD8 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). Anti-CD8 antibody (32-M4) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CD8 protein, also known as Leu 2 or T8, is a crucial cell surface glycoprotein that forms a two-chain complex (either αα or αβ) and plays a vital role in the immune response by binding to class I major histocompatibility complex (MHC) molecules presented by antigen-presenting cells (APCs). This interaction is essential for the activation of T cytotoxic cells, which are responsible for directly killing infected or cancerous cells. CD8 enhances the avidity of T cell receptor (TCR) interactions with antigens, thereby facilitating effective immune responses. CD8-expressing T cells may contribute to the maintenance of low levels of HIV expression, highlighting CD8′s importance in both adaptive immunity and viral control.

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CD8 Antibody (32-M4) References:

1. Potentiating the antitumour response of CD8(+) T cells by modulating cholesterol metabolism.  |  Yang, W., et al. 2016. Nature. 531: 651-5. PMID: 26982734
2. Epigenetic control of CD8+ T cell differentiation.  |  Henning, AN., et al. 2018. Nat Rev Immunol. 18: 340-356. PMID: 29379213
3. Cholesterol Induces CD8+ T Cell Exhaustion in the Tumor Microenvironment.  |  Ma, X., et al. 2019. Cell Metab. 30: 143-156.e5. PMID: 31031094
4. TOX transcriptionally and epigenetically programs CD8+ T cell exhaustion.  |  Khan, O., et al. 2019. Nature. 571: 211-218. PMID: 31207603
5. The Roles of CD8+ T Cell Subsets in Antitumor Immunity.  |  St Paul, M. and Ohashi, PS. 2020. Trends Cell Biol. 30: 695-704. PMID: 32624246
6. Memory CD8+ T cell responses to cancer.  |  Han, J., et al. 2020. Semin Immunol. 49: 101435. PMID: 33272898
7. Systemic vaccination induces CD8+ T cells and remodels the tumor microenvironment.  |  Baharom, F., et al. 2022. Cell. 185: 4317-4332.e15. PMID: 36302380
8. CD8+ T cell exhaustion and cancer immunotherapy.  |  Wang, Q., et al. 2023. Cancer Lett. 559: 216043. PMID: 36584935
9. Regulation of CD8+ T memory and exhaustion by the mTOR signals.  |  Chen, Y., et al. 2023. Cell Mol Immunol. 20: 1023-1039. PMID: 37582972
10. Circadian tumor infiltration and function of CD8+ T cells dictate immunotherapy efficacy.  |  Wang, C., et al. 2024. Cell. 187: 2690-2702.e17. PMID: 38723627

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CD8 Antibody (32-M4) has 31 citations in a variety of scientific publications.

Primary antibodies like Anti-CD8 Antibody (32-M4) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CD8 Antibody (32-M4) Specifications

Alternate Product Name: CD8

Product Clone Name: 32-M4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids:

Epitope Target: CD8

Epitope Species: human

Product Reactivity/Detection: CD8

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, FCM

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 31 citations in various scientific publications

Available Conjugates/Forms for CD8 Antibody (32-M4): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, APC: Allophycocyanin, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CD8 antibody is CD8 Antibody (UCH-T4): sc-1181.