CD8-alpha Antibody (KT15): sc-53473

CD8-α Antibody (KT15) is a rat monoclonal IgG2a antibody that specifically detects the CD8-α chain of mouse origin by immunofluorescence (IF) and flow cytometry (FCM). Raised against T cell clone C6 of mouse origin, anti-CD8-α antibody (KT15) targets CD8-α, also known as CD8A or T8, a transmembrane glycoprotein expressed on the surface of cytotoxic T lymphocytes and natural killer cells. CD8-α forms either homodimers (CD8αα) or heterodimers with CD8-β (CD8αβ) and plays a crucial role in the immune response by interacting with major histocompatibility complex (MHC) class I molecules presented by antigen-presenting cells (APCs). This interaction enhances the recognition of antigenic peptides by the T cell receptor (TCR) complex, which includes CD3 proteins, and facilitates signal transduction necessary for T cell activation. CD8-α associates with the Src family tyrosine kinase Lck through its cytoplasmic tail, which is essential for downstream signaling pathways leading to T cell proliferation and cytokine production. Understanding the interactions between CD8-α and its associated proteins is important for research into immune responses, autoimmune diseases, and immunotherapies, making CD8-α monoclonal antibody (KT15) a valuable tool for studying these interactions in mouse models.

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CD8-alpha Antibody (KT15) References:

1. Familial CD8 deficiency due to a mutation in the CD8 alpha gene.  |  de la Calle-Martin, O., et al. 2001. J Clin Invest. 108: 117-23. PMID: 11435463
2. CD8 alpha alpha-mediated intraepithelial lymphocyte snatching of thymic leukemia MHC class Ib molecules in vitro and in vivo.  |  Pardigon, N., et al. 2006. J Immunol. 177: 1590-8. PMID: 16849467
3. Recurrent infections and bilateral uveitis in a patient with CD8 deficiency.  |  Farhoudi, A., et al. 2005. Iran J Allergy Asthma Immunol. 4: 43-5. PMID: 17301423
4. Analysis of the functional capabilities of CD3+CD4-CD8- and CD3+CD4+CD8+ human T cell clones.  |  Patel, SS., et al. 1989. J Immunol. 143: 1108-17. PMID: 2526180
5. Identification and analysis of the expression of CD8 alpha beta and CD8 alpha alpha isoforms in chickens reveals a major TCR-gamma delta CD8 alpha beta subset of intestinal intraepithelial lymphocytes.  |  Tregaskes, CA., et al. 1995. J Immunol. 154: 4485-94. PMID: 7722305
6. The CD8 coreceptor interaction with the alpha 3 domain of HLA class I is critical to the differentiation of human cytotoxic T-lymphocytes specific for HLA-A2 and HLA-Cw4.  |  Wesley, PK., et al. 1993. Hum Immunol. 36: 149-55. PMID: 8320133
7. CD8 inhibits signal transduction through the T cell receptor in CD4-CD8- thymocytes from T cell receptor transgenic mice reconstituted with a transgenic CD8 alpha molecule.  |  van Oers, NS., et al. 1993. J Immunol. 151: 777-90. PMID: 8335907
8. Non-tolerant B cells cause autoimmunity in anti-CD8 IgG2a-transgenic mice.  |  Battegay, M., et al. 1996. Eur J Immunol. 26: 250-8. PMID: 8566075
9. The cytoplasmic domain of CD8 beta regulates Lck kinase activation and CD8 T cell development.  |  Irie, HY., et al. 1998. J Immunol. 161: 183-91. PMID: 9647223
10. Early stages in the development of human T, natural killer and thymic dendritic cells.  |  Spits, H., et al. 1998. Immunol Rev. 165: 75-86. PMID: 9850853

The preferred CD8-α antibody is CD8-α Antibody (53-6.7): sc-18913.