CD72 Double Nickase Plasmid (h): sc-404054-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD72 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD72 Double Nickase Plasmid (h) and CD72 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CD72. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD72 Antibody (G-5): sc-25265
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD72 Double Nickase Plasmid (h) | sc-404054-NIC | 20 µg | $410.00 | |||
CD72 Double Nickase Plasmid (h2) | sc-404054-NIC-2 | 20 µg | $410.00 |
CD72 is a B cell–restricted type II transmembrane glycoprotein that functions as an immunoregulatory co-receptor, coupling antigen receptor signals to intracellular inhibitory pathways. Through immunoreceptor tyrosine-based inhibitory motif (ITIM) phosphorylation and recruitment of phosphatases such as SHP-1/SHIP, CD72 modulates BCR-driven activation thresholds, calcium flux, and downstream MAPK and PI3K signaling. CD72 also binds the semaphorin family ligand SEMA4D (CD100), influencing B cell–T cell interactions, germinal center responses, and peripheral tolerance. Dysregulated CD72 signaling has been associated with altered B cell homeostasis and autoimmunity, and its expression and pathway context are frequently examined in B cell malignancy biology and immune microenvironment studies.
CD72 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CD72 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CD72. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CD72 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CD72-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.