CD63 Double Nickase Plasmid (h): sc-400061-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CD63 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CD63 Double Nickase Plasmid (h) and CD63 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CD63. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CD63 Antibody (E-12): sc-365604
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD63 Double Nickase Plasmid (h) | sc-400061-NIC | 20 µg | $410.00 | |||
CD63 Double Nickase Plasmid (h2) | sc-400061-NIC-2 | 20 µg | $410.00 |
CD63 (tetraspanin-30) is a widely expressed tetraspanin enriched on late endosomes, lysosomes, and exosomes, where it helps organize tetraspanin-enriched microdomains that coordinate membrane protein trafficking and signaling. By partnering with integrins and immune receptors, CD63 regulates endosomal maturation, vesicle docking and fusion, antigen processing, and cell adhesion–related pathways. CD63 is commonly used as a marker of extracellular vesicles and is implicated in processes such as immune modulation, viral entry/egress, and regulation of protease activity in secretory granules. Altered CD63 expression or localization has been associated with inflammatory responses and cancer cell invasion/metastasis phenotypes, motivating mechanistic studies of its role in membrane dynamics and intercellular communication.
CD63 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CD63 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CD63. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CD63 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CD63-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.