CD5 Antibody (L17F12): sc-18898

CD5 Antibody (L17F12) is a mouse monoclonal IgG2a antibody that detects CD5 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and flow cytometry (FCM). CD5 is a crucial transmembrane glycoprotein predominantly expressed on T lymphocytes, accounting for approximately 70% of normal peripheral blood lymphocytes and nearly all T cells in the thymus and peripheral blood. CD5 plays a significant role in T cell activation; upon engagement of the T cell receptor (TCR), CD5 undergoes tyrosine phosphorylation, which is essential for modulating T cell responses. Notably, the absence of CD5 leads to hyper-responsiveness of T cells to TCR-mediated activation, highlighting CD5′s importance in maintaining immune homeostasis. CD5 interacts with the TCR/CD3-ζ chain and the Src family kinase Lck, enhancing Lck′s kinase activity by 10- to 15-fold when bound to CD5, which is critical for effective T cell signaling. Additionally, CD5 serves as a receptor for the B cell antigen CD72, although the precise consequences of this interaction remain to be fully elucidated, suggesting a potential role in thymic selection processes. Anti-CD5 antibody (L17F12) is an invaluable tool for researchers studying T cell biology and immune regulation.

The preferred CD5 antibody is CD5 Antibody (UCH-T2): sc-1180.