CD5 Antibody (H65): sc-53205

CD5 Antibody (H65) is a mouse monoclonal IgG1 antibody that detects CD5 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). CD5 monoclonal antibody (H65) recognizes a crucial transmembrane glycoprotein predominantly expressed on T lymphocytes, where CD5 plays a significant role in modulating T cell activation and survival. Located on the surface of these immune cells, CD5 interacts with the T cell receptor (TCR) and the CD3-ζ chain, facilitating a complex signaling cascade that influences T cell responses. Notably, CD5 phosphorylation upon TCR engagement enhances the activity of the Src family kinase Lck, leading to a 10- to 15-fold increase in kinase activity, which is vital for effective T cell signaling. Furthermore, CD5′s interaction with the B cell antigen CD72 suggests a potential role in thymic selection, although the exact consequences of this binding remain an area of active research. Anti-CD5 antibody (H65) accurately detects and characterizes CD5, making this reagent an invaluable tool for studying T cell biology and the immune response.

The preferred CD5 antibody is CD5 Antibody (UCH-T2): sc-1180.