CD45RB Antibody (16A): sc-18846
- CD45RB Antibody (16A) is a rat monoclonal IgG2a CD45RB antibody, cited in 2 publications, provided at 200 µg/ml
- raised against clone TH2 mouse cells expressing CD45RB
- CD45RB Antibody (16A) is recommended for detection of CD45RB of mouse origin by WB, IP, IF, IHC(P) and FCM
- Anti-CD45RB Antibody (16A) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CD45RB Antibody (16A). This work is in progress.
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CD45RB Antibody (16A) is a rat monoclonal IgG2a antibody engineered to target the CD45RB isoform of the CD45 protein, also known as Protein Tyrosine Phosphatase Receptor Type C (PTPRC). CD45RB is a vital transmembrane glycoprotein expressed on the surface of all nucleated hematopoietic cells in mice, playing a crucial role in regulating immune cell signaling and activation. Structurally, CD45RB consists of an extracellular domain generated through alternative splicing of exons 4, 5, and 6, a single transmembrane region, and a conserved intracellular domain that possesses phosphatase activity. This structural organization is essential for CD45RB′s function, as the variability in the extracellular domain allows for differential interactions with other cell surface molecules, thereby modulating signal transduction pathways critical for T and B cell receptor signaling. Located on the cell membrane, CD45RB dephosphorylates key tyrosine residues on Src family kinases such as Lck and Fyn, as well as on Syk family kinases like ZAP-70, which are essential for initiating and sustaining signaling cascades that drive lymphocyte development, activation, and immune responses. Additionally, CD45RB undergoes significant post-translational modifications, including extensive glycosylation, which are crucial for its stability, proper localization on the cell membrane, and interactions with other signaling proteins, thereby enhancing its regulatory functions. Anti-CD45RB antibody (16A) is raised against clone TH2 mouse cells expressing CD45RB and is effective in various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM) across mouse, rat, and human samples. CD45RB monoclonal antibody (16A) facilitates comprehensive analysis of immune cell populations and their interactions with key signaling proteins, providing valuable insights into immune system dynamics and the mechanisms underlying immune-related diseases. CD45RB (16A) antibody′s ability to detect multiple isoforms of CD45RB, resulting from alternative splicing, allows researchers to investigate the diversity and function of different immune cell subsets, making CD45RB (16A) monoclonal antibody an indispensable tool for both research and diagnostic purposes in immunology and hematology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CD45RB Antibody (16A) | sc-18846 | 200 µg/ml | $322.00 | |||
CD45RB Antibody (16A) AC | sc-18846 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CD45RB Antibody (16A) HRP | sc-18846 HRP | 200 µg/ml | $322.00 | |||
CD45RB Antibody (16A) FITC | sc-18846 FITC | 200 µg/ml | $336.00 | |||
CD45RB Antibody (16A) PE | sc-18846 PE | 200 µg/ml | $349.00 | |||
CD45RB Antibody (16A) Alexa Fluor® 488 | sc-18846 AF488 | 200 µg/ml | $364.00 | |||
CD45RB Antibody (16A) Alexa Fluor® 546 | sc-18846 AF546 | 200 µg/ml | $364.00 | |||
CD45RB Antibody (16A) Alexa Fluor® 594 | sc-18846 AF594 | 200 µg/ml | $364.00 | |||
CD45RB Antibody (16A) Alexa Fluor® 647 | sc-18846 AF647 | 200 µg/ml | $364.00 | |||
CD45RB Antibody (16A) Alexa Fluor® 680 | sc-18846 AF680 | 200 µg/ml | $364.00 | |||
CD45RB Antibody (16A) Alexa Fluor® 790 | sc-18846 AF790 | 200 µg/ml | $364.00 |